中文摘要：

目的探讨肿瘤坏死因子a诱导蛋白3交互作用蛋白1（TNFα induced protein 3 interactingprotein 1, TNIP1）在基因和蛋白质水平对胸腺瘤患者重症肌无力（myasthenia gravis，MG）发病的影响。方法术前采集11例重症肌无力合并胸腺瘤（myastheaia gravis associated thymoma, MGT）患者和11例非重症肌无力的胸腺瘤（non-myasthenia gravis thymoma, NMGT）患者胸腺瘤新鲜组织标本，应用实时荧光定量PCR及Western印迹技术检测TNIP1 mRNA及蛋白表达水平；术前采集所有病例的静脉血，术后3～6个月采集MGT患者静脉血，应用实时荧光定量PCR和Western印迹检测TNIP1 mRNA和蛋白质的表达量。结果MGT患者胸腺瘤组织TNIP1 mRNA和蛋白表达水平均显著低于NMGT患者；MGT患者的外周血单个核细胞TNIP1mRNA和蛋白表达水平显著低于NMGT患者（P〈0．05）；术后3～6月MGT患者外周血单个核细胞中TN伊1mRNA和蛋白表达水平较术前明显增高。结论TNIP1表达量降低可能参与胸腺瘤患者重症肌无力的发生、发展过程，胸腺切除手术有助于纠正TNIP1的表达异常。

英文摘要：

Objective To explore the role of TNFa induced protein 3 interacting protein 1 （TNIP1） in the pathogenesis of myasthenia gravis （MG） among patients with thymoma. Methods From December 2014 to March 2015, 11 patients with MG associated thymoma （MGT） and 11 non-MG thymoma （NMGT） patients receiving thymectomy were selected. Thymus specimens were obtained during surgery, and peripheral venous blood samples were obtained before the surgery. For the MGT cohorts, peripheral venous blood samples were also collected at 3-6 months after the surgery. TNIPJ mRNA was determined with quantitative real-time PCR （qPCR）, and its protein expression was evaluated by Western blotting. Results For both thymus specimen and peripheral blood samples, the levels of TNIP1 mRNA and protein in the MGT group were both significantly lower than those of the NMGT patients （P〈0.05）. For the MGT group, the levels of TNIP1 mRNA and protein of the peripheral blood samples have increased following thymus resection compared with before the surgery. Conclusion Reduced TNIP1 expression may have a role in the pathogenesis of MG for patients with thymoma. Thymectomy may help to recover the expression of TNIP1 among such patients.