中文摘要：

以三苯甲烷类染料乙基紫为研究对象,研究了Kurthia huakuii LAM0618T重组酚氧化酶LaclK对乙基紫的脱色性能,探讨了给酶量、脱色时间、染料浓度、反应p H、卤离子、有机试剂以及NO-3、SO2-4和腐殖酸对脱色效果的影响。在pH 7.0,60℃条件下反应1 h,200 U/L的LaclK对10μmol/L和20μmol/L的乙基紫显示出较好的脱色效果,脱色率分别达到91%和84%。此外,LaclK能够耐受卤离子和有机试剂,具有一定的生物修复应用潜力。

英文摘要：

The dye decolorization ability of recombinant Lacl K on ethyl violet was studied in this work. Effects of parameters,such as amount of Lacl K,incubation time,ethyl violet concentration,p H,halide ions,organic solvents,NO-3,SO2-4 and humic acid on the decolorization was investigated. After incubation at 60℃ and pH 7. 0 for 1 h,200 U / L of Lacl K decolorized 91% of 10 μmol / L ethyl violet and 84% of 20 μmol / L ethyl violet,respectively. Moreover,Lacl K exhibited a strong tolerance to halide ions and organic solvents,indicating the industrial application potential on bioremediation.