中文摘要：

目的 探讨骨髓间质干细胞（BM-MSCs）修复顺铂诱导的急性肾损伤的分子机制。方法 以6 mg/kg顺铂的剂量经腹腔注射大鼠体内,24 h后经尾静脉输注BM-MSCs（BM-MSCs组）或PBS（PBS组）,以未注射顺铂者作为正常对照组;HE染色及免疫组织化学染色法检测BM-MSCs对肾损伤的修复情况;体外培养NRK-52E细胞并经顺铂作用6 h后,继续培养48 h（顺铂组）或与BM-MSCs共培养48 h（细胞组）,未用顺铂处理的NRK-52E细胞作为对照组;qRT-PCR检测其miR-92b及其靶基因PTEN的表达水平,western blot检测其p-Akt蛋白的表达水平。结果 HE染色结果显示,BM-MSCs组的肾小管蛋白质管型明显少于PBS组,肾小管结构明显改善;免疫组织化学染色结果表明,BM-MSCs组的增殖细胞核抗原（PCNA）阳性细胞数[（131.0±14.4）个]明显高于PBS组[（42.2±6.1）个],差异有统计学意义（t=11.28,P〈0.01）;qRT-PCR结果表明,体内试验中,与正常对照组miR-92b和PTEN基因的表达水平（1.11±0.78,1.01±0.21）相比,PBS组分别为4.64±1.06和0.61±0.2,差异有统计学意义（P〈0.05）,BM-MSCs组分别为2.27±0.81和1.1±0.1,差异亦有统计学意义（P均〈0.05）;体外实验中,与阴性对照组miR-92b和PTEN基因的表达水平（1.12±0.77,1.02±0.13）相比,顺铂组分别为7.64±0.72和0.58±0.2,差异有统计学意义（P均〈0.05）,细胞组分别为4.38±0.50和1.15±0.23,差异亦有统计学意义（P均〈0.05）;western blot检测结果显示,与顺铂组p-Akt蛋白的表达水平（0.96±0.18）相比,细胞组p-Akt蛋白表达水平为2.11±0.11,差异有统计学意义（P〈0.01）。结论 BM-MSCs能够修复顺铂诱导的急性肾损伤,可能通过下调miR-92b发挥作用。

英文摘要：

Objective To investigate the molecular mechanism of bone marrow mesenchymal stem cells （BM-MSCs） in repairing cis- platin-induced acute renal injury. Methods The rats were injected 6 mg/kg of cisplatin intraperitoneally, and bone marrow mesen- chymal stem cells （BM-MSCs group） or PBS （PBS group） were injected respectively via tail vein after 24 hours. The rats without in- jecting cisplatin were selected as a normal control group. The repair effect of BM-MSCs on renal injury was observed by HE staining and immunohistochemistry. In addition, NRK-52E cells were cultured in vitro and treated with cisplatin for 6 hours. Then, NRK-52E cells were continued to culture for 48 hours or co-cultured with BM-MSCs for 48 hours, and NRK-52E cells untreated with cisplatin were used as a control. The expression levels of miR-92b and its target gene PTEN were detected by qRT-PCR, and the expression lev- el of p-Akt by western blot. Results HE staining showed that the tubular protein casts in BM-MSCs group were significantly less than that in PBS group, and that the renal tubular structure was significantly improved in BM-MSCs group. Immunohistochemical staining indicated that the number of ceils expressing proliferating cell nuclear antigen （PCNA） in BM-MSCs group （ 131.0 ± 14.4） was signif- icantly higher than that in PBS group （42.2 ± 6.1, t = 11.28, P 〈 0.01 ）. qRT-PCR results showed that in the vivo experiment, com- pared with the expression level of miR-92b and PTEN in the normal control group （ 1. 11 ± 0.78,1.01 ± 0.21 ）, PBS group were （4.64 ± 1.06） and （0.61 ± 0.2）, respectively （ all P 〈 0.05 ） ; BM-MSCs group were （2.27 ± 0.81 ） and （ 1.1 ± 0.1 ）, respectively （all P 〈 0.05 ）. In vitro experiment, compared with the expression level of miR-92b and PTEN in the negative control group （1.12 ± 0.77,1.02 ± 0.13 ）, cisplatin group were （7.64 ± 0.72） and （0.58 ± 0.2）, respectively （ all P 〈 O. 05 ）, cell group were （4.38 ± 0.50） and （1