中文摘要：

目的：观察钠‐钾‐氯共转运体1（Na＋‐K＋‐2 Cl－cotransporter 1，NKCC1）抑制剂布美他尼（Bumetanide）对脑微血管内皮细胞（BM EC ）缺氧损伤后增殖的影响。方法将原代培养的大鼠BM EC分为3组：对照组、氧糖剥夺／复氧（OGD／Reo ）组和OGD／Reo＋Bumetanide（10μmol／L ）组。在氧糖剥夺／复氧6、12、24和48 h时，用M T T法检测细胞活力，Edu染色以及流式细胞术检测细胞增殖及细胞周期情况。结果氧糖剥夺／复氧6、12、24 h ，BM EC活力下降，Edu染色阳性率减少且S期细胞减少。而与OGD／Reo组相比，OGD／Reo＋Bumetanide组BM EC活力增加[复氧6 h：（0.497±0.039） v s.（0.612±0.044）]，Edu染色阳性率增加[6 h时：（5.742±0.195）％ v s.（7.125±0.144）％]，且S期细胞增多[6 h时：（5.845±0.389）％ v s.（7.124±0.542）％]，差异均有统计学意义（均 P＜0.05）。结论布美他尼可促进缺氧损伤后BMEC的活力及细胞增殖，提示NKCC1通路在BMEC缺氧损伤后的细胞增殖中发挥了重要作用。

英文摘要：

Objective To observe the effects of Bumetanide（NKCC1 inhibitor）on proliferation of brain microvascular endo‐thelial cells（BMEC）after oxygen glucose deprivation/reoxygenation（OGD/Reo）.Methods Primary BMEC were randomly di‐vided into three groups ：control group ,OGD/Reo group and OGD/Reo＋Bumetanide group. At different time points（6 ,12 ,24 , 48 h）of reoxygenation ,cell vitality was determined by MTT test.Edu dyeing and flow cytometry were used to detect the cell cy‐cle progress.Results After intervention with OGD/Reo for 6 ,12 and 24 h ,BMEC viability ,Edu positive rate and cells in S phase were decreased.Compared with the OGD/Reo group ,the cell vitality ,Edu positive rate and cells in S phase were signifi‐cantly increased [（0.497 ± 0.039） vs. （0.612 ± 0.044） ,（5.742 ± 0.195）% vs. （7.125 ± 0.144）% ,（5.845 ± 0.389）% vs. （7.124 ± 0.542）% ,all P＆lt; 0.05].Conclusion Bumetanide can increase BMEC vitality and promote cell proliferation after OGD ,suggesting NKCC1 plays an important role in proliferation of BMEC after OGD.