中文摘要：

目的：研究知母及其各化学拆分组分的抗炎免疫活性及其物质基础的对应关系。方法：C57小鼠除正常对照组外均采用2,4二硝基氟苯诱发超敏反应模型,观察小鼠免疫器官指数、耳肿胀度、耳组织IL-4及IFN-γ含量,体外实验观察刀豆蛋白（Con A）诱导的小鼠脾淋巴细胞增殖力、脂多糖（LPS）诱导小鼠巨噬细胞RAW264.7炎症因子分泌量。结果：知母煎液组、皂苷组及多糖组小鼠耳肿胀度及免疫器官指数显著降低（P〈0.05）,免疫细胞增殖能力显著降低（P〈0.01）,小鼠巨噬细胞炎症因子NO、TNF-α分泌显著减少（P〈0.05）。结论：知母具有显著的抗炎及免疫调节活性,其物质基础为皂苷组分和多糖组分。

英文摘要：

Objective： To study the relationship between chemical subdivision and immune suppressive activity in order to find out the anti-inflammatory and immunomudulatory pharmacological activity material basic of Anemarrhenae Rhizoma. Method： C57 mice was induced by 2,4-Dinitrofluorobenzene to build immune inflammation which was also called contact allergic dermatitis. The influence of Anemarrhenae Rhizoma decoction and chemical subdivisions on immune organ,ear edema and the Th1/Th2 immune balance was measured by analytical balance and Elisa method. The inflammatory factor TNF-α and NO level excreted by macrophage RAW264. 7 induced by LPS were also investigated. Result： AA decoction,timosaponin and polysaccharides significantly reduced the immune organ index and ear edema degree（ P〈0. 05）,protein expression of IFN-γ was inhibited by AA timosaponin fraction and polysaccharides fraction. In vitro experiments showed that the proliferation of spleen cells was inhibited by timosaponin and polysaccharides after induced by Con A（ P〈0. 05）. The release of NO and TNF-α induced by LPS significantly decreased by Anemarrhenae Rhizoma decoction and timosaponin（ P〈0. 05）. Conclusion： The significant anti-inflammatory and immunomudu latory effects of AA are related to timosaponin and polysaccharides.