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Lhx8在海马胆碱能神经再生中的作用
  • ISSN号:0529-1356
  • 期刊名称:解剖学报
  • 时间:2013.12.12
  • 页码:721-734
  • 分类:R322.8[医药卫生—人体解剖和组织胚胎学;医药卫生—基础医学]
  • 作者机构:[1]南通大学医学院人体解剖学系,江苏省神经再生重点实验室,江苏南通226001
  • 相关基金:国家自然科学基金资助项目(31070937);江苏高校优势学科建设工程资助项目(PAPD)
  • 相关项目:Lhx8在海马胆碱能神经再生中的作用
中文摘要:

目的探讨切割穹隆海马伞大鼠海马胆碱能神经再生过程中Lhx8的作用。方法72只SD大鼠随机分成4组,每组18只。所分的4组分别为:对照组(未做任何处理),切割组(切割右侧穹隆海马伞),过表达组(右侧海马齿状回中注射Lenti6.3-Lhx8慢病毒)及切割联合过表达组(右侧海马齿状回中注射Lenti6.3-Lhx8慢病毒并切割右侧穹隆海马伞)。28d后每组各取6只分别制备脑冷冻切片行免疫荧光检测,提取海马总RNA行Real—time PCR,提取海马蛋白行Western blotting检测。结果与对照组相比,切割组、过表达组和切割联合过表达组中Lhx8mRNA和蛋白水平均显著上调,以切割联合过表达组中上调最为显著,组间比较差异均有统计学意义;切割组胆碱乙酰转移酶(CHAT)mRNA水平与对照组相比无统计学差异,过表达组和切割联合过表达组中ChAT mRNA显著上调,且以切割联合过表达组中上调最为显著;切割组、过表达组和切割联合过表达组中均检测到ChAT蛋白,组间比较差异有统计学意义;切割组、过表达组和切割联合过表达组海马齿状回门区和颗粒下层中均能检测到新生的Lhx8阳性的胆碱能神经元,且以切割联合过表达组最多,组间比较差异均有统计学意义。结论上调Lhx8的表达能促进海马中神经干细胞向胆碱能神经元分化,Lhx8可能与海马胆碱能神经再生有关。

英文摘要:

Objective To investigate the effect of Lhx8 in hippocampal cholinergic neural regeneration of SD rat after fimbria-fornix transection. Methods According to the different treatments, 72 SD rats were randomly divided into four groups: the normal group, the transected group, the overexpressed group and the transected combined with overexpressed group. On the 28th day after treatments, the sections of rat hippocampus of each group were prepared and stained with immunohistochemieal methods to detect the number of newborn Lhx8 and ChAT positive cells. PCR and Western blotting were performed after total RNA and protein of hippocampus were obtained respectively. Results Compared with the normal group, the levels of Lhx8 mRNA and protein were significantly increased in other three groups and the differences were significant among the groups. No significant difference of the levels of ChAT mRNA was observed between the normal group and the transeeted group while it was significantly upregulated in the overexpressed and the transected combined with overexpressed groups. ChAT protein was detected in the transected, overexpressed and the transected combined with overexpressed groups and the differences were significant among the groups. There were newborn Lhx8 positive cholinergic neurons in hilus and subgranular zone of hippocampal dentate gyrus in the transected, overexpressed and the transected combined with overexpressed groups, with the most in the transected combined with overexpressed group, and the differences were significant among the groups. Conclusion Upregulation of Lhx8 can promote differentiation of neural stem cells into cholinergic neurons in hippocampus significantly, and Lhx8 may be related to cholinergic neural regeneration of hippocampus.

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期刊信息
  • 《解剖学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科协
  • 主办单位:中国解剖学会
  • 主编:章静波
  • 地址:北京海淀区学院路38号北京大学医学部
  • 邮编:100191
  • 邮箱:jpxb@bjmu.edu.cn
  • 电话:010-82802969
  • 国际标准刊号:ISSN:0529-1356
  • 国内统一刊号:ISSN:11-2228/R
  • 邮发代号:2-249
  • 获奖情况:
  • 1992年中国科协优秀学术期刊三等奖,1997年第二届全国优秀科技期刊二等奖,97、98、99连续三年科技基础性和高科技期刊资助三等奖,中国期刊方阵“双百”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),荷兰文摘与引文数据库,荷兰医学文摘,美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:9672