中文摘要：

目的 观察大鼠脑内不同脑区，不同低温条件下冷诱导RNA结合蛋白（CIRP）mRNA的表达情况。方法 利用麻醉配合体表降温，制作亚低温大鼠模型。将大鼠分为对照组、低温30min、低温1h、低温2h、低温4h组，每组5只。保持低温状态恒定后，在相应时间点处死大鼠．取下丘脑、海马、皮层脑组织。以PT—PCR半定量检测各脑区在不同低温时间点时CIRP mRNA的表达。结果 正常体温下，海马CIRP mRNA的表达相对最强，皮层居中，下丘脑最弱。低体温1h后，CIRP mRNA的表达在下丘脑首先增高；低体温2h后．海马和皮层的CIRP mRNA的表达才开始增高，此时下丘脑CIRP mRNA的表达未见变化：低体温4h后，海马CIRP mRNA的表达较2h时仍有所增高，而下丘脑和皮层则保持不变。结论 不同脑区CIRP mRNA的表达在低温下均明显增高，各脑区表达不一，提示这种CIRP的高表达可能参与了低温下机体对脑损伤的保护作用。

英文摘要：

Objective To observe the expression of cold-inducible RNA-binding protein （CIRP） mRNA in different brain regions in rats under different hypothermic conditions. Methods Mild hypothermic models were established in rats through the combined application of anesthesia and body surface cooling with alcohol. Rats were divided into 5 groups： control, 30 min hypothermia, 1 h hypothermia, 2 h hypothermia and 4 h hypothermia（n=5 in each group）. The body temperature of rats was kept stable and rats were executed at corresponding time points. Then they were decapitated and their hypothalamic, hippocampal and cortical brain tissues were removed. The expression of CIRP mRNA in different brain regions was measured at different time points of hypothermia by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）. Results The expression of CIRP mRNA was the strongest in hippocampus and the lowest in hypothalamus under normothermia. After 1 h hypothermia, the expression of CIRP mRNA in hypothalamus was firstly increased; after 2 h hypothermia, its expression in hippocampus and cortex began to increase but the expression remained unchanged in hypothalamus. After 4 h hypothermia, its expression in hippocampus was higher than that at 2 h, but the expressions still maintained their previous levels in hypothalamus and cortex. Conclusion Expressions of CIRP mRNA have all been increased significantly in different brain regions of rats under hypothermic conditions and the expression varies among different brain regions, indicating that the high expression of CIRP mRNA might participate in the hypothermic protection of the brain against injury.