从健康雷公藤植株体内分离得到23株内生细菌,对其有益生物学特征及对小麦萌发能力的影响进行评价,得出3株益生效果显著的内生细菌.这3株内生细菌均能产生植物生长素和铁载体,其中LG3、LJ10具有溶磷特性,LG3、LY4具有固氮功能并同时具有1-氨基环丙烷-1-羧酸脱氨酶(ACC脱氨酶)活性.经细菌16SrDNA序列鉴定及同源性分析发现,LG3、LY4属于肠杆菌属,U10属于泛菌属.回接试验表明,3株内生细菌不但能够促进雷公藤植株的生长,还能显著提高不同器官内雷公藤甲素的含量.
A total of twenty-three endophytic bacteria were isolated from Tripterygium wilfordii, among which three strains were selected for further studying based on their capabilities of growth- promotion and wheat germination. All three isolated strains could produce phytohormone and siderophore, and LG3 and LJ10 strains were capable of dissolving phosphorus. Additionally, LG3 and LY4 strains were both proved to have nitrogen-fixing function and 1-aminocyclopropane-1- carboxy-late deaminase activity. Bacterial 16S rDNA sequence identification and homology analysis suggested that LG3 and LY4 strains belonged to the Enterobacter and LJ10 belonged to the Pantoea. The results of reinoculation experiment demonstrated that three endophytic bacteria could not only promote the growth of Tripterygium wilfordii, but also improve the triptolide contents of different organs significantly.