中文摘要：

目的 筛选新的卵巢特异性蛋白质,探讨其可能在卵细胞成熟及胚胎发育过程中作用.方法 收集小鼠成熟卵细胞,经二维电泳、蛋白印迹、质谱鉴定,获得新的多肽序列;应用RT、RACE-PCR等技术,获得该段多肽对应的新蛋白的全长cDNA序列,并行Northernblot分析其组织特异性.结果 所获得的多肽序列来自一种未知新蛋白,其对应的cDNA全长有1510 bp,前32 bp为5＇非编码区,第33～1433 bp为ORF区,第1434～1510 bp为3＇非编码区;该基因定位于9号染色体上,预测表达466个氨基酸,理论相对分子质量和pI分别为53 400和8.05,具有稳定WD40,F-box保守性功能域,并特异性表达于卵巢组织.结论 一种具有卵巢组织特异性的新蛋白被发现,其生物信息学特征表明其可能参与卵细胞内分子信号通道,可能在卵成熟及早期胚胎发育中具有作用.

英文摘要：

Objective To screen and identify the novel ovarian specific proteins to explore the possible mechanisms in the oogenesis and early embryo development. Methods The oocytes of BALB/C mice were collected for 2-D electrophoresis. After reaction with anti-citrulline antibody, three positive spots were selected for mass spectrometry. Based on the biologic informatic analysis, a novel peptide sequence was found and specific primers were designed for cloning. A full-length cDNA was identified by reverse transcription and rapid amplification of cDNA ends - polymerase chain reaction （ RACE-PCR ） . Its nucleotide and possible protein sequences were compared with those in the gene bank. And the tissuespecific characteristic of MOP53 was analyzed by Northern blot. Results A novel peptide sequence was found in murine oocytes, it came from a predicted protein with 330 amino acids. The predicted protein had ～53 400 and pI 8. 05 with conserved WD40, F-box motif. A full-length cDNA sequence with 1510 bp was cloned and named MOP53. Located at chromosome 9 F2, this new gene was specifically expressed in ovary.Conclusions An ovarian specific protein is found. It may be involved in the process of oogenesis and early embryo development based on its biologic informatic characteristics.