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大麦SSR标记遗传多样性及其与农艺性状关联分析
  • ISSN号:0578-1752
  • 期刊名称:《中国农业科学》
  • 时间:0
  • 分类:S635.1[农业科学—蔬菜学;农业科学—园艺学]
  • 作者机构:[1]甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室,兰州730070, [2]甘肃农业大学农学院,兰州730070, [3]甘肃农业大学生命科学技术学院,兰州730070
  • 相关基金:国家自然科学基金面上项目(30771331,31171558); 国家大麦青稞产业技术体系(CARS-05)
中文摘要:

【目的】分析大麦亲本材料的遗传多样性,寻找与部分农艺性状相关联的分子标记,为大麦杂交组合的配置及分子标记辅助育种提供依据。【方法】利用86个SSR标记对113份大麦亲本材料进行多态性扫描,并进行遗传多样性分析。挑选57个标记进行群体遗传结构分析,在此基础上采用Tassel 2.1 GLM(general linearmodel)和MLM(mixed linear model)方法进行标记与农艺性状的关联分析。【结果】86个标记共检测出200个等位变异,变异范围为1—5个;基因频率的变异范围为0.0088—1.0000,Shannon指数变异范围为0.0000—1.2236;遗传相似系数(GS)变异范围在0.5504—0.9897,平均值为0.7477。通过群体遗传结构分析将供试材料分为4个亚群。以GLM分析,发现9个与株高、穗长、芒长、穗粒数和小穗着生密度相关联的标记,各标记对表型变异的解释率在0.0507—0.2766;以MLM分析,发现6个与株高、芒长和小穗着生密度相关的标记,各标记对表型变异的解释率在0.0238—0.1999。【结论】利用SSR标记分析了113份大麦亲本材料的遗传多样性及群体遗传结构,并通过2种关联分析模型,分别寻找到了9个与株高、穗长、芒长、穗粒数相关联,6个与株高、芒长和小穗着生密度相关联的标记,这些标记位于1H、2H、3H、4H和7H染色体。

英文摘要:

[Objective] In order to provide useful information for hybridization combination of parent materials and molecular assisted breeding, the genetic diversity of parent materials was determined by using SSR markers and their association with some agronomic traits were detected. [ Method ] A total of 86 SSR markers were screened for polymorphism among parent materials, and then the analysis on genetic diversity of these materials were detected. Population structure was analyzed through 57 selected markers, and then association analysis between SSR markers and 5 agronomic traits were performed using TASSEL GLM (General Linear Model) and MLM (Mixed Linear Model) programs. [Result] A total of 200 alleles were found from 113 materials, ranged from l to 5. Allele frequency ranged from 0.0088 to 1.0000 and Shannon index ranged from 0.0000 to 1.2236. The genetic similarity ranged from 0.5504 to 0.9897, with the mean of 0.7477. Genetic structure analysis showed that the population of these parent materials was composed of 4 subpopulations. There were 9 SSR markers associated with plant height, spike length, awn length, grain number per spike and Spikelet Compactness under GLM program, and the rate of explanation on the phenotype of related marker ranged from 0.0507 to 0.2766. There were 6 SSR markers associated with plant height, awn length and spikelet compactness under MLM program, and the rate of explanation on the phenotype of related marker ranged from 0.0238 to 0.1999. [ Conclusion ] Genetic diversity and population structure of 113 materials were analyzed through SSR markers and their association with phenotypes were detected. Nine markers, associated with plant height, spike length, awn length, grain number per spike and spikelet compactness, were determined under GLM program. And 6 markers associated with plant height, awn length and spikelet compactness were determined under MLM program. These markers were on chromosomes 1H, 2H, 3H, 4H and 7H, respectively.

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期刊信息
  • 《中国农业科学》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院 中国农学会
  • 主编:万建民
  • 地址:北京中关村南大街12号中国农业科学院图书馆楼4101-4103室
  • 邮编:100081
  • 邮箱:zgnykx@caas.cn
  • 电话:010-82109808 82106279
  • 国际标准刊号:ISSN:0578-1752
  • 国内统一刊号:ISSN:11-1328/S
  • 邮发代号:2-138
  • 获奖情况:
  • 中国期刊方阵“双高”期刊,第三届中国出版政府奖提名奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:85620