综述了在废水生物除磷系统分子生物学分析方法中应用较为普遍的变性梯度凝胶电泳、荧光原位杂交、16SrDNA克隆文库技术的基本原理与使用中存在的主要问题,对其在废水生物除磷系统种群结构分析中的应用加以阐述,并展望了在废水生物除磷系统中分子生物学技术的应用前景和发展方向。
The basic principles and main problems existing in denaturing gradient gel electrophoresis, fluo- rescence in situ hybridization and 16SrDNA cloning library, which wildely used in wastewater biological phosphorus removal system, were reviewed. The application of molecular biology technology for polyphosphate population structure analysis of wastewater biological phosphorus removal system were expatiated, further more, its application prospects and development direction in the said field were also looked forward.