中文摘要：

建立了基质固相分散萃取-高效液相色谱-串联质谱法（MSPD-HPLC-MS/MS）同时测定拟南芥中3种赤霉素GA1,GA3和GA4的分析方法。将拟南芥样品与C18填料混合研磨制成MSPD柱,并采用80%冷甲醇洗脱。采用反相C18色谱柱进行分离,以0.05%甲酸水溶液和乙腈为流动相进行梯度洗脱,采用电喷雾离子源负离子模式（ESI-）电离,多反应监测模式检测。对样品前处理条件、色谱分离条件和质谱检测条件进行了优化,结果表明,在最优条件下,3种赤霉素在10～300 ng/g范围内均呈良好线性关系,相关系数（r2）均大于0.98,检出限在1.1～4.1 ng/g之间。在10～50 ng/g添加水平下,平均回收率范围为54.7%～102.6%,相对标准偏差（RSD,n=3）为3.2%～12.8%。该方法操作简单、灵敏度高、选择性好、回收率高,适合拟南芥中GA1、GA3、GA4含量的测定。

英文摘要：

A method for the analysis of gibberellin A1（GA1）,gibberellin A3（GA3） and gibberellin A4（GA4） in Arabidopsis thaliana by matrix solid-phase dispersion extraction（MSPD） and high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） was developed.The solid sample of Arabidopsis thaliana was gently blended with C18 to obtain a homogeneous mixture.This mixture was transferred to an SPE cartridge filled with 0.5 g C18 to form a MSPD column.GA1,GA3 and GA4 were eluted with cold 80% methanol aqueous solution.The target compounds were separated on a C18 column with a gradient elution of 0.05% formic acid aqueous solution and acetonitrile as the mobile phase.The identification and quantification were carried out by using electrospray ionization in negative ion mode（ESI-） with multiple reaction monitoring（MRM）.The linear ranges for GA1,GA3 and GA4 were all from 10 to 300 ng/g with correlation coefficients greater than 0.98.The limits of detection were in the range of 1.1-4.1 ng/g.The average recoveries and relative standard deviations were 54.7%-102.6% and 3.2%-12.8% respectively in the spiked range of 10-50 ng/g.The method is simple,sensitive,efficient and accurate.It is suitable for the confirmation and quantitative determination of GA1,GA3 and GA4 in Arabidopsis thaliana.