中文摘要：

目的制备聚乙二醇（PEG）转化生长因子（TGF）-β1缓释微球去细胞瓣复合支架，观察生物学性能。方法制备聚乙二醇微球，电镜观察粒径。去细胞瓣与PEG微球耦联，吸附TGF—β1，行酶联免疫吸附试验（ELISA）检测，形态学与分子生物学检测。种植大鼠肌成纤维细胞，体外培养7d，构建组织工程心脏瓣膜（TEHV），行形态学与分子生物学检测。结果PEG微球粒径（42．72±3．48）nm。ELISA检测示TGF—β1包封率82．01％，7dTGF—β1释放率67．22％。与去细胞瓣支架比较，复合支架组细胞紧密联合且细胞外基质丰富，羟脯氨酸含量和DNA含量增高。结论PEG—TGF—β1缓释微球去细胞瓣复合支架，利于细胞生长，胶原的分泌及TEHV的构建。

英文摘要：

Objective To prepare decelluarized valve scaffolds modified by polyethylence glycol （PEG） microspheres loading transforming growth factor-β1 （TGF-β1）, and observe its biological properties. Methods PEG microspheres were produced by an emulsion-crosslinking method,and scanning electron microscopic observation was performed to examine the average diameter. Decelluarized valve scaffolds were modified with PEG microspheres ,then TGF-β1 was loaded into them by adsorption. Controlled release of TGF-β1 was measured by ELISA, and histomorphology observation and molecular biological detection were performed. The myofibroblasts harvested from rats were seeded on the scaffolds. After culture for one week in vitro, histomorphology observation and molecular biological detection were performed. Results The average diameter of PEG microspheres was （ 42.72 ＋ 3.48 ） nm. The percentage of cumulative release was 67.22% within 7 days, and the encapsulation eMciency was 82.01%. Compared with the simple decelluarized valve scaffolds,the modified scaffolds showed that cells contacted closely and extracellular matrix were more bloomy, and DNA and hydroxyproline contents were also increased. Conclusion Decelluarized valve scaffolds modified by PEG microspheres loading TGF-β1 are beneficial to cell proliferation, secretion of collagen, and the construction of TEHV.