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Optimizing the Quadruple-potential Waveform for the Determination of Gentamicin Sulfate by High Performance Liquid Chromatography with Pulsed Electrochemical Detection
  • ISSN号:1001-604X
  • 期刊名称:《中国化学:英文版》
  • 时间:0
  • 分类:O646.5[理学—物理化学;理学—化学]
  • 作者机构:[1]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
  • 相关基金:Project supported by the Major State Basic Research Development Program (No. 2003CB415001) of China and the National Natural Science Foundation of China (No. 20475060).
中文摘要:

在这份报纸,四倍潜力的波形被颠倒的阶段离子对层析为 gentamicin 的决心调查并且优化。而不是一个相对高的积极潜力,一个否定潜力为清洗金工作电极作为一个潜力被采用。由这样,在清洗的 analyte 察觉和电极期间源于高积极的潜力的应用程序的金氧化物的形成极大地被减少,并且因此,金工作电极的溶解和衰退也被减少。工作电极由这四倍潜力的波形完成了的黄金的好条件能帮助我们获得好重制度。以便尽可能高获得 signal-to-noise 比率,影响 gentamicin 的察觉的几个波形参数小心地被选择。分析方法与低相对标准差被用于二件真实 gentamicin 样品,和好结果的决心不超过 4% 被获得。

英文摘要:

In this paper, a quadruple-potential waveform was investigated and optimized for the determination of gentamicin by reversed phase ion-pair chromatography. Instead of a relatively high positive potential, a negative potential was adopted as a potential for the cleaning of gold working electrode. By this way, the formation of gold oxide resulting from the application of high positive potential during the analyte detection and electrode cleaning was greatly reduced, and therefore, the dissolution and recession of gold working electrode was also reduced. The good condition of gold working electrode achieved by this quadruple-potential waveform can help us to obtain a good reproducibility. In order to acquire signal-to-noise ratio as high as possible, several waveform parameters affecting the detection of gentamicin were carefully selected. The analytical method has been applied to the determination of two real gentamicin samples, and good results with low relative standard deviation not more than 4% were obtained.

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期刊信息
  • 《中国化学:英文版》
  • 主管单位:
  • 主办单位:中国化学会
  • 主编:
  • 地址:上海市枫林路354号中科院上海有机化学研究所
  • 邮编:200032
  • 邮箱:
  • 电话:021-54925243
  • 国际标准刊号:ISSN:1001-604X
  • 国内统一刊号:ISSN:31-1547/O6
  • 邮发代号:4-646
  • 获奖情况:
  • 中国期刊方阵“双高”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),荷兰文摘与引文数据库,美国科学引文索引(扩展库),日本日本科学技术振兴机构数据库,英国英国皇家化学学会文摘
  • 被引量:175