中文摘要：

目的将与焦点的服的 ischemic 灌注损害在 Wistar 老鼠调查 baicalin 的 neuro 保护的效果。称 320350 g 的 90 只成年男 Wistar 老鼠随机被划分成下列组(n=5 ) 的方法：(a) 假冒的控制组；(b) 车辆组，使遭到了到中间的服的动脉吸藏并且 intraperitoneally 收到了车辆；(c-e ) baicalin 组织 25， 50 和 100，它受到中间的服的动脉吸藏并且与 baicalin 对待 mg/kg 分别地。神经病学的分数被决定在手术后在处理以后的 1， 3 和 7 d。激活朊酶的 receptor-1 (PAR-1 ) 的表示， PAR-1 mRNA 和 Caspase-3 分别地用西方的污点，反向的抄写聚合酶链反应(RTPCR ) 分析和 immunohistochemistry 被决定。结果重要减少与车辆组的相比在 baicalin 组在神经病学的分数被注意(P < 0.01 ) 。另外， PAR-1 mRNA， PAR-1 和 Caspase-3 的下面规定与从车辆组获得的那些相比在 baicalin 组被观察(P < 0.01 ) 。与低剂量的 baicalin 组(25 mg/kg ) 相比，显著减少在高剂量的组在 PAR-1 mRNA， PAR-1 以及 Caspase-3 的神经病学的分数，和表示被注意(P < 0.05 ) 。结论 Baicalin 通过禁止 PAR-1 和 apoptosis 的表示在焦点的服的 ischemic 灌注损害显示出 neuro 保护的效果。

英文摘要：

Objective： To investigate the neuro-protective effects of baicaiin in Wistar rats with focal cerebral ischemic reperfusion injury. Methods： Ninety adult male Wistar rats weighing 320-350 g were randomly divided into the following groups （n=5）： （a） sham control group; （b） vehicle group, subjected to middle cerebral artery occlusion and received vehicle intraperitoneally; （c-e） baicalin groups, which were subjected to the middle cerebral artery occlusion and treated with baicalin 25, 50 and 100 mg/kg, respectively. The neurological scores were determined at postoperative 1, 3 and 7 d after the treatment. The expression of protease-activated receptor-1 （PAR-1）, PAR-1 mRNA and Caspase-3 were determined using Western blot, reverse transcription polymerase chain reaction （RT- PCR） analysis and immunohistochemistry, respectively. Results： Significant decrease was noted in the neurological score in the baicalin group compared with that of the vehicle group （P〈0.01）. Additionally, down-regulation of PAR-1 mRNA, PAR-1 and Caspase-3 was observed in the baicalin groups compared with those obtained from the vehicle group （P〈0.01）. Compared with the low-dose baicalin group （25 mg/kg）, remarkable decrease was noted in neurological score, and the expression of PAR-1 mRNA, PAR-1 as well as Caspase-3 in the high-dose group （P〈0.05）. Conclusion： Baicalin showed neuro-protective effects in focal cerebral ischemic reperfusion injury through inhibiting the expression of PAR-1 and apoptosis.