中文摘要：

目的：探讨乳腺癌细胞中HuR调控PDGFC的分子机制.方法：通过软件预测分析,乳腺癌细胞中PDGFC 3＇UTR的HuR结合位点;在RNA免疫共沉淀实验中,加入PDGFC刺激后检测HuR与PDGFC mRNA的相互作用;通过构建PDGFC 3＇UTR五个截短体,荧光素酶报告基因实验检测HuR调控PDGFC的结合位点.结果：软件预测分析发现,PDGFC 3＇UTR可能存在五个HuR结合位点;RNA免疫共沉淀实验中,当加入PDGFC刺激后,HuR与PDGFCmRNA出现免疫共沉淀,证明HuR和PDGFC之间的直接相互作用;PDGFCmRNA3＇UTR报告基因系统检测显示,第2个和第4个位点可与HuR结合调控PDGFC.结论：本研究揭示了乳腺癌细胞中HuR通过与PDGFC mRNA 3＇UTR结合调控PDGFC的分子机制,为乳腺癌的临床诊断和治疗提供了新的思路.

英文摘要：

Objective：To investigate the molecular mechanism of PDGFC regulated by HuR in breast cancer cells.Methods：Through the software analysises,we first predicted the HuR-binding sites on the PDGFC 3＇-UTR in breast cancer cells;An RNA-immunoprecipitation tested the interaction of HuR with the PDGFC mRN after adding PDGFC stimulation;Luciferase experiments tested the HuR-binding sites of PDGFC regulated by HuR through structuring five truncated of PDGFC 3＇UTR.Results：We found five HuR-binding sites in the 3＇UTR of PDGFC by software prediction;The RNA-immunoprecipitation showed the co-immunoprecipitation of HuR and PDGFC mRN after adding PDGFC stimulation confirming the direct association of HuR with the PDGFC;Luciferase experiments ofPDGFC mRNA 3＇UTR showed that PDGFC regulated by the second and fourth HuR-binding sites.Conclusions：This study reveals the molecular mechanism of PDGFC regulated by HuR through binding to PDGFC mRNA 3＇UTR in breast cancer cells,and provided rationale for the development of strategies in the clinical diagnosis and treatment for breast cancer. ABSTRACT Objective： To investigate the molecular mechanism of PDGFC regulated by HuR in breast cancer cells. Methods： Through the software analysises, we first predicted the HuR-binding sites on the PDGFC 3＇-UTR in breast cancer cells; An RNA-im- mtmoprecipitation tested the interaction of HuR with the PDGFC tuRN after adding PDGFC stimulation; Luciferase experiments tested the HuR-binding sites of PDGFC regulated by HuR through structuring five truncated of PDGFC 3＇UTR. Results： We found five HuR-binding sites in the 3＇UTR of PDGFC by software prediction; The RNA-immunoprecipitation showed the co-immunoprecipitation of HuR and PDGFC tuRN after adding PDGFC stimulation confirming the direct association of HuR with the PDGFC; Luciferase experi- ments ofPDGFC mRNA 3＇UTR showed that PDGFC regulated by the second and fourth HuR-binding sites. Conclusions： This study re- veals the molecular mechanism of PDGFC regulated by Hu