从生物陶粒反应器中筛选出6株异养硝化细菌。采用乙酸钠-氯化铵培养基培养细菌进行硝化特性研究,经过12d好氧培养,6株异养硝化细菌对COD的去除率在45%以上,总氮和氨氮最终去除率在60%以上,并且具有产生NOx-N的硝化性能.应用PCR—DGGE(denaturing gradienr gel eleleetrophoresis)方法研究了SBR反应器种群结构动态变化规律,PCR—DGGE图谱分析表明,微生物多样性与废水的处理效果出现协同变化的特征,1d与15d微生物群落结构相似程度最高为75%,1d与30d相似性最低为52.94%,15d与30d相似性为70.59%,3株筛选的异氧硝化细菌wgy1、wgy3、wgx5成为SBR反应器的优势菌群.
6 heterotrophic nitrobacteria are screened from bio-ceramic reactors. And sodium acetate and ammonium chloride are used as carbon and nitrogen source to foster bacteria for the characteristic study of nitrification. COD removal rate could reach over 45 %, TN and NH4-N removal rate could reach over 60 %, and also it has the nitrification function of producing NOx-N. By the method of PCR-DGGE, this paper studies the dynamic changes of the SBR reactor. The PCR-DGGE profiles show that shift of microbial diversity corresponds to the effect of nitrate removal. 1 d and the 15 d similarity is 75%, which microbial diversity is the highest. 1 d and the 30 d similarity is the lowest which is just 52.94%, 15 d and the 30 d. similarity is 70.59 %. wgy1, wgy3 and wgx5 are dominated bacteria in sequencing batch reactor.