中文摘要：

目的研究转化生长因子-β1（TGF-β，）和Smad6在支气管哮喘（简称哮喘）大鼠肺组织中表达变化及福莫特罗对其表达的影响。方法无特定病原体级（SPF）健康雄性SD大鼠60只，根据干预方法和时间分为对照组（A2和A4），哮喘组（B2和B4），福莫特罗组（C2和C4），每组10只。用卵白蛋白（ovalbumin，OVA）致敏与激发，建立哮喘大鼠气道重塑模型，福莫特罗组模型制作同哮喘组，在激发阶段，激发前30min给予福莫特罗灌胃处理．各组均在末次激发后1～2h处死大鼠。采用图像分析技术测定肺内支气管壁厚度（Wat）及支气管平滑肌厚度（Wam）；免疫组化法测定肺组织中TGF-β1及Smad6蛋白表达。结果（1）Wat：02组和B2组比较差异无统计学意义（P〉0.05），两者均显著厚于A2组（均P〈0．01），C4组显著薄于B4组（P〈0．01），两者均显著厚于A4组（均P〈0．01）；（2）Wam：C2组显著薄于B2组（P〈0.01），两者均显著厚于A2组（均P〈0.01），C4组显著薄于B4组，两者均显著厚于A4组（均P〈0．01）；（3）肺组织TGF-β1蛋白平均吸光度值：C2组低于B2组（P〈0．05），两者均显著高于A2组（均P〈001），C4组低于B4组（均P〈0．05）。两者均显著高于A4组（均P〈0．01）；（4）肺组织Smad6蛋白平均吸光度值：C2组与B2组比较差异无统计学意义（P〉0．05），两者均显著低于A2组（均P〈0．01），C4组显著低于B4组（P〈0．01），两者均显著高于A4组（均P〈0．01）。结论TGF-β1在哮喘气道重塑大鼠肺组织中表达增高，Smad。表达减低；TGF一13，和Smad。表达失衡可能促进了哮喘气道重塑的发生和发展；福莫特罗可抑制哮喘大鼠TGF-β1，表达，促进Smad。表达，作用随激发时间延长而增强。

英文摘要：

Objective To investigate the expression of TGF- β1 and Smad6 in airway remodeling of asthmatic rats and the regulation of formoterol. Methods Sixty SPF grade male SD rats were divided into 6 groups with 10 in each; the asthma model was induced by sensitization with ovalbumin. A2w and A4w were assigned as control groups; asthma was induced in group B2w and B4w; the asthmatic rats in group C2w and C4w were given with lmg/kg formoterol gavage 30 rain before the excitation. Animals in all groups were sacrificed 1-2h after last excitation. The thickness of total brochial wall （Wat） and the thickness of airway smooth muscle （Warn） were measured by image analysis system. The expression of TGF- β1 and Smad6 was detected by immunohistochemistry technique. Results There were no differences in Wat between C2w [（34.55 ± 3.70） μm2 m] and B2w[ （48.19 ± 4.75） μm2/μm2 （P〉0.05）, but Wat in C2w and B2 w was all thicker than that of A2w[（29.92 ± 2.33） μm2/μm] （P〈 0.01）; Wat in C4w[ （51.09 ± 2.96） μm2/μm] was significantly thinner than that in B4w[（56.82 ± 4.64） μm2/μ m] （P 〈 0.01）, both were significantly thicker than that in A4w [（31.80 ±2.81） μm2/μm] （P〈0.01）. The Warn of C2w [ （16.53 ± 1.94） μm2/μm] was significantly thinner than that in B2w [（18.79 ±2.18） μm2/μm] （P〈0.01）, both were thicker than that in A2w [（12.87 ± 1.58） μm2/m] （P〈 0.01）, Warn in C4w[ （19.21 ± 1.40） μm2/μm] was significantly thinner than B4w[（22.10 ± 1.99） μm2/μm] （P〈 0.01）, both were thicker than that in A4w [（12.96 ± 0.61）μm2/μm] （P 〈 0.01）. The expression of TGF- β1 in C2w[ （0.25 ± 0.02）] was lower than that in B2w [（0.27 ±0.02）] （P〈 0.05）, both were significantly higher than that in A2w[（0.18±0.02）] （P〈0.01）; the expression of C4w [（0.27 ± 0.03）] was lower than B4w [（0.30 ± 0.03）]（P 〈 0.05）, both were significantly higher than that in A4w[ （0.19± 0.02）] （P 〈 0.01 ?