中文摘要：

目的分析microRNA-30a（miR-30a）在胃癌中的表达水平并鉴定其新的靶基因。方法选取29例胃癌患者的肿瘤组织和癌旁组织,利用qRT-PCR检测miR-30a的表达。通过miRNA靶基因预测数据库Target Scan预测miR-30a的靶基因。构建含有miR-30a结合位点的3＇UTR片段的荧光素酶载体（p MIR-FAPα）,通过荧光素酶报告实验对预测靶基因进行检测。利用Western blot和qRT-PCR对预测靶基因成纤维活化蛋白α（fibroblast activation proteinα,FAPα）的表达进行检测。结果 miR-30a在胃癌组织样本中的表达明显下调,通过生物信息学预测（Target Scan）和荧光素酶实验表明miR-30a mimic可与FAPα基因结合,qRT-PCR和Western blot结果表明miR-30a mimic可抑制FAPαmRNA和蛋白质水平的表达。结论 miR-30a在人胃癌组织样本中表达下调,FAPα是其靶基因。

英文摘要：

Objective To investigate the expression of microRNA-30a （miR-30a） in human gastric cancer （GC） tissues and identify its direct target gene. Methods The expression of miR-30a in the tumor tissues and paratumor tissues of 29 patients with GC was detected by qRT-PCR. The potential target genes of miR-30a were predicted with TargerScan database. Luciferase reporter plasmid pMIR-fibroblast activation protein α （FAPα） with FAPα 3′UTR as the binding site of miR-30a was constructed. The predicted target gene （FAPα） of miR-30a was identified by dual luciferase activation assay. The mRNA and protein expression levels of FAPα were assessed by qRT-PCR and Western blotting, respectively. Results The expression of miR-30a was significantly decreased in the tumor tissues compared with the paratumor tissues. Low expression of miR-30a in the tumor tissues was significantly correlated with the clinical stage. MiR-30a mimic significantly inhibited luciferase activation （P〈0.05） in the HEK293 cells, which proved that miR-30a could target FAPα 3′UTR. Western blotting and qRT-PCR showed that miR-30a inhibited the mRNA and protein expression levels of FAPα. Conclusion MiR-30a is down-regulated in GC, and FAPα is its target gene.