中文摘要：

目的通过检测miR-155和miR．146a在类风湿关节炎（RA）患者外周血单个核细胞（PBMC）和血浆中的表达，探讨miR-155和miR-146a表达在RA发病中的作用。方法RA患者34例，其中高活动组23例，低活动组11例；健康对照组15名。取外周血分离PBMC和血浆，提取和纯化miRNA，实时定量聚合酶链反应（PCR）检测miR-155和miR-146a的表达，以小分子RNAU6作为内对照，计算标准化后的2-值表示miRNA的相对表达含量。采用SPSS13．0软件包进行方差齐性分析和t检验。结果RA患者PBMC和血浆miR-155的表达分别是对照组的1．74倍（0．08_＋0．08与0．05±0．03，t=-2．225，P〈0．05）和4．65倍（5．9±6．7与1.3±2．0，￡：-3．677，P〈0．05）；miR-146a的表达分别是对照组的1．68倍（1-3±1．2与0．8±0．6，t=-2．154，P〈0．05）和2．47倍（741±1001与300±295，t=-1．669，P〉0．05）；RA患者中高活动组和低活动组PBMC中miR-155和miR-146a的表达差异无统计学意义；而血浆miR-155和miR-146a的表达高活动组明显高于低活动组，差异有统计学意义（P〈0．05）。结论miR-155和miR．146a在RA患者PBMC和血浆中异常高表达，且血浆miR．155和miR-146a的表达与RA活动度相关，提示血浆rniR-155和miR-146a可以作为RA及RA活动度的预测指标。

英文摘要：

Objective To investigate the expression of miR-155 and miR-146a in peripheral blood mononuclear cells （PBMC） and plasma of rheumatoid arthritis （RA） patients. Methods PBMC and plasma were separated from the peripheral blood of 34 RA patients and 15 healthy individuals. Total RNAs were isolated and miRNAs were purified. The levels of miR-155 and miR-146a were determined by quantitative reverse transcription PCR （qRT-PCR）. U6 was used as housekeeping control. The amount of target miRNA was normalized relative to the amount of U6 （ACt=ACt,ACtu6）. Relative expression levels were expressed as Data were analyzed using SPSS 13.0 software. The test of homogeneity of variance and unpaired t-test was used to compare between groups. P values （2-tailed） less than 0.05 were considered as statistically significant. Results The expressions of PBMC and plasma miR-155 were higher in RA patients than those in the healthy control individuals （0.08±0.08 vs 0.05±0.03, t=-2.225, P〈0.05; 5.9±6.7 vs 1.3±2.0, t=-3.677, P〈 0.05 ）. The expression of miR-146a in PBMC and plasma of RA patients and controls were （1.3：el.2 vs 0.8± 0.6, t=-2.154, P〈0.05）and （741±1001 vs 300±295, t=-1.669, P〉0.05）. According to their DAS28 value, RA patients were divided into high activity group （23 cases, DAS28/〉 5.0） and low disease activity group（ 11 cases, DAS28〈5.0）. The plasma miR-155 and miR-146a expressions were significantly higher in high activitygroup than those in low activity group. There were no significant differences in the expression of PBMC miR- 155 and miR-ld6a between the two groups. Conclusion The expression of PBMC and plasma miR-155 and miR-146a are higher in RA patients. The expression of plasma miR-155 and miR-146a are associated with RA patients＂ activity. Plasma miR-155 and miR-146a may be potential non-invasive biomarkers for RA diagnosis anddisease activity assessment.