中文摘要：

目的比较Van Gieson氏染色和Masson氏三色染色法用于日本血吸虫感染小鼠肝脏纤维化染色的效果。方法建立日本血吸虫感染小鼠肝脏组织纤维化模型,取肝脏标本,常规切片,进行Van Gieson氏染色和Masson氏三色染色,应用图像分析软件统计虫卵肉芽肿纤维化面积。结果染色时间为3～7 min时,2种方法显示肉芽肿纤维化面积差异无统计学意义（P〉0.05）;染色时间为10 min时,由于非特异性因素使得Masson氏染色法着色面积明显大于Van Gieson氏染色法,两者差异有统计学意义（P〈0.05）。结论Van Gieson氏染色法比Masson氏三色操作过程相对简单,易于掌握,染色结果稳定、可靠,可以作为评价日本血吸虫感染小鼠肝脏纤维化程度的较好染色方法。

英文摘要：

Objective To compare the effects of collagen fiber staining between Van-Gieson staining and Masson trichrome staining of hepatic specimens in mice with Schistosoma japonicum infection.Methods A model of hepatic granuloma and fibrosis was established by infecting mice with S.japonicum cercariae,then the hepatic specimens were taken and Van-Gieson staining and Masson trichrome staining were performed.Eventually,the area of granuloma and fibrosis were measured by imaging analysis software.Results When the time of staining was 3-7 min,there was no significant difference of the fibrosis areas between the two methods（P0.05）;when the time of staining was more than 10 min,the staining area showed by Masson＇s staining was significantly larger than that showed by Van-Gieson staining,and the difference was statistically significant（P0.05）.Conclusion The operation procedures of Van-Gieson staining are simpler and easier to master than those of Masson trichrome staining,therefore Van-Gieson staining is a better method to display collagen.