中文摘要：

目的探讨酢浆草总提取物对酒精性肝病大鼠的治疗作用及其分子机制。方法将42只雄性 SD 大鼠按随机数字表法分为正常对照组（n＝10）、模型组（n＝8）、酢浆草中剂量组（n＝8）、酢浆草高剂量组（n＝8）和泼尼松组（n＝8）。采用白酒灌胃的方法制备酒精性肝病大鼠模型；制模12周后，酢浆草中、高剂量组和泼尼松组分别给予酢浆草总提取物3.5 g·kg-1·d-1、7 g·kg-1·d-1或醋酸泼尼松0.9 mg·kg-1·d-1，其余两组每日灌胃等量生理盐水，共6周。检测各组大鼠肝功能、超氧化物歧化酶（SOD）、丙二醛（MDA）及肝组织乙醇脱氢酶（ADH）和乙醛脱氢酶（ALDH）水平；光镜下观察各组大鼠肝组织病理形态学变化；免疫组化染色检测肿瘤坏死因子-α（TNF-α）的表达水平。结果与正常对照组比较，模型组天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（AKP）、γ-谷氨酰转移酶（GGT）、MDA 含量均明显升高，SOD、ADH 和ALDH 含量均明显降低；与模型组比较，各药物组AST、ALT、AKP、GGT、MDA 含量均明显降低，SOD、ADH 和ALDH 均明显升高，AST、ALT、AKP、GGT、SOD、ADH 以酢浆草高剂组变化更显著〔 AST（U/L）：117.38±22.75比201.62±17.95，ALT（U/L）：33.51±11.64比59.14±9.52，AKP（U/L）：95.19±24.85比169.39±37.21，GGT （U/L）：46.54±14.55比89.37±12.49，SOD（U/mg）：137.03±12.03比80.64±13.45，ADH（U/L）：3.48±0.71比2.05±0.91，P＜0.05或P＜0.01〕，MDA、ALDH 以酢浆草中剂组变化更显著〔 MDA（mmol/mg）：2.05±0.64比3.17±0.61，ALDH（U/L）：7.59±1.95比5.71±1.33，均P＜0.05〕。正常对照组大鼠肝组织未见明显病变；模型组肝细胞以大泡性为主的脂肪变性；酢浆草中、高剂量组大泡性脂肪变细胞明显减少。免疫组化染色结果显示，酢浆草中、高剂量均可显著降低TNF-α在肝组织中巨噬细胞胞质及部分细?

英文摘要：

Objective To investigate the therapeutic action and molecular mechanisms of Oxalis comiculata L. extracts on rats with alcoholic liver disease. Methods Forty-two male Sprague Dawley（SD）rats were randomly divided into normal control group（n=10）,model group（n=8）,moderate dose oxalis group（n=8）,high dose oxalis group（n=8）and prednisone group（n=8）. The model of rat with alcoholic liver disease was established by liquor gavage;after 12 weeks,moderate dose oxalis group,high dose oxalis group and prednisone group were given the total extract of oxalis 3.5 g？kg-1？d-1,7 g？kg-1？d-1 or prednisolone acetate 0.9 mg？kg-1？d-1,respectively,the remaining two groups were given the same amount of normal saline by gavage daily for 6 weeks. Levels of indexes of liver function,superoxide dismutase（SOD）,malondialdehyde（MDA）,antidiuretic hormone（ADH）and aldehyde dehydrogenase（ALDH）in rats were detected. The pathological changes of liver tissues in rats were observed under light microscope;tumor necrosis factor-α（TNF-α） expression level was detected by immunohistochemical staining. Results Compared with the normal control group, the levels of aspartate aminotransferase （AST）, alanine aminotransferase（ALT）,alkaline phosphatase（AKP）,γ-glutamyl transferase（GGT）,MDA were increased significantly,while the levels of SOD,ADH and ALDH were obviously reduced in model group. Compared with the model group,AST,ALT,AKP,GGT and MDA contents were decreased significantly,while the levels of SOD, ADH and ALDH were markedly increased in the drug groups,and the changes of levels of AST,ALT,AKP,GGT, SOD,ADH in high dose oxalis group were the most obvious〔AST（U/L）：117.38±22.75 vs. 201.62±17.95,ALT （U/L）：33.51±11.64 vs. 59.14±9.52,AKP（U/L）：95.19±24.85 vs. 169.39±37.21,GGT（U/L）：46.54±14.55 vs. 89.37±12.49,SOD（U/mg）：137.03±12.03 vs. 80.64±13.45,ADH（U/L）：3.48±0.71 vs. 2.05±0.91,P〈0.05 or P〈0.01〕,and the most significant changes of MD