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中文摘要：
为了鉴定广东湛江地区菠萝心腐病病原菌，研究了病原菌的致病性、菌丝体及孢子囊等形态特征．分析了核糖体DNA—ITS序列同源性。结果表明，该病原菌属于疫霉属真菌，同源性分析显示，该菌与GenBank中烟草疫霉ITS序列的同源性达99％以上；采用烟草疫霉特异性引物进行PCR检测，结果确认该病原菌为烟草疫霉菌（Phytophthoranicotianae）。
英文摘要：
In order to identify the pathogen of pineapple leaf heart-rot disease in Guangdong, the fungi was isolated from diseased pineapples. Based on the morphological characteristics, pathogenicity and the sequence of ribosomal DNA-ITS, the pathogen was identified as Phytophthora nicotianae, and the homology analysis showed the sequence of ribosomal DNA-ITS isolated from BL1 had homology of more than 99% with Phytophthora nicotianae published in GenBank. Finally, PCR detection using P. nicotianae specific orimers indicated that the. nathn~,~n w~ P nr~otlanaa
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