中文摘要：

目的：观察母鼠孕前和孕期高脂饮食对新生仔鼠骨骼生长的影响，并探讨影响骨骼生长的机制。方法40只雌性Sprague-Dawley大鼠随机分为高脂组和对照组（n=20），分别喂养35%高脂饲料和普通饲料。喂养8周后，高脂组和对照组各取8只雌鼠观察其肝脏组织病理；其余与普通饲料喂养的雄性大鼠交配，孕期分别继续给予高脂饲料或普通饲料喂养。待娩出新生仔鼠后24 h内，测量两组仔鼠体长（鼻尾长度）；酶联免疫吸附试验测定血清胰岛素样生长因子-I（IGF-I）水平；光镜下观察肝脏组织病理；免疫组织化学法检测长骨（胫骨、股骨）中胰岛素受体底物1（IRS-1）和磷酸化IRS-1（Phospho-IRS-1）的表达；蛋白质印迹技术检测长骨软骨细胞中促分裂原活化蛋白激酶（MAPK）和磷酸化MAPK（Phospho-MAPK）、磷酯酰肌醇3-激酶（PI3K）和磷酸化PI3K（Phospho-PI3K）、蛋白激酶B（AKT1）和磷酸化AKT1（Phospho-AKT1）的蛋白表达。结果高脂组仔鼠的出生体长较对照组显著降低（P〈0.05）。高脂组仔鼠血清IGF-I水平较对照组下降，但差异无统计学意义（P〉0.05）。高脂组母鼠及仔鼠肝组织可见脂肪样变，而对照组肝脏病理形态正常。两组仔鼠长骨软骨细胞中IRS-1的表达差异无统计学意义（P〉0.05）。高脂组仔鼠长骨软骨细胞中MAPK的表达水平高于对照组（P〈0.05），而PI3K及AKT1/Phospho-AKT1的表达水平在两组间差异无统计学意义（P〉0.05）。结论母鼠孕前和孕期高脂饮食会影响胎鼠在宫内的骨骼发育，可能与IGF-I的下降有关，但未发现IGF-I对骨骼影响的确切发病机制。

英文摘要：

Objective To explore the mechanism and effect of maternal high-fat diet before and during pregnancy on bone growth of neonatal offspring rats. Methods Forty female Sprague-Dawley rats were divided into high-fat diet and control groups （n=20） that were fed with 35%high-fat diet and standard chow, respectively. After 8 weeks, 8 female rats from each group were sacriifced for liver pathological examinations and the other female rats were mated with male rats and fed continuously with 35%high-fat diet and standard chow throughout gestation, respectively. The body lengths （from apex nasi to end of tail） of the offspring rats from both groups were measured within 24 hours after birth. Enzyme-linked immunosorbent assay was used to detect serum insulin-like growth factor （IFG-I） levels. Liver pathological changes were observed under a light microscope. The expression of insulin receptor substrate 1 （IRS-1） and phosphorylation IRS-1 （Phospho-IRS-1） in tibia and femur samples were detected by immunohistochemistry. The expression of mitogen-activated protein kinase （MAPK） and phosphorylation MAPK （Phospho-MAPK）, phosphatidylinositol 3-kinase （PI3K） and phosphorylation PI3K （Phospho-PI3K）, protein kinase B （AKT1） and phosphorylation AKT1 （Phospho-AKT1） in tibia and femur samples were detected by Western blot. Results The offspring rats from the high-fat diet group showed a significant shorter body length compared with those from the control group （P〈0.05）. The level of serum IGF-I in offspring rats from the high-fat diet group decreased by 20.1%in comparison to those from the control group, but there was no signiifcant difference between the two groups （P〉0.05）. Fatty degeneration was found in livers of both high-fat diet-fed maternal rats and their offspring rats under a light microscope. There were no signiifcant differences in IRS-1 and Phospho-IRS-1 expression in chondrocytes of tibia and femur samples between the offspring rats of the two groups （P〉0.05）. The