中文摘要：

目的：观察慢性低压性缺氧和重组鼠白介素-1β（rmlL-1β）刺激对大鼠颈动脉体（carotid body，CB）中磷酸化细胞外信号调节蛋白激1／2（p-ERKI／2）表达的影响。方法：雄性sD大鼠分为8组，分别为缺氧刺激0、1、2、3周组和缺氧0、1、2、3周的同时伴maiL-1β刺激组。对cB进行免疫组化染色，并用Western Blot法对p-ERK1／2进行半定量分析。结果：相对于缺氧0周组，缺氧2周和缺氧3周组大鼠CB中p-ERKI／2的含量明显增加。相对于单纯给予缺氧，缺氧同时给予maiL-1β刺激后引起大鼠CB体中p-ERKI／2表达量的增加。结论：慢性缺氧和rmIL-1β刺激均可致颈动脉体p-ERK1／2上调；慢性缺氧伴rmIL-1β刺激比单纯缺氧或rmIL-1β刺激可引起P-ERK1／2更显著的增加。

英文摘要：

Objective： To observe the effects of chronic hypoxia and systemic administration of recombinant marine in- terleukin-1 beta（rmIL-1β） on the expression of phosphorylated extracelluar sigual-regulated kinase1/2 （ p-ERK1/2 ） in the rat carotid body（CB）. Methods： Adult male Sprague-Dawley rats were divided into 8 groups： chronic intermittent hypobaric oxygen stimulation for 0, 1, 2 and 3 weeks groups, and chronic intermittent hypobaric oxygen exposure for 0, 1,2, and 3 weeks paired with rmIL-1βinjection groups. Immunohistochemistry and Western Blot techniques were em- ployed to observe expressions of p-ERK1/2 in the CB of all rats. Results： The expression of p-ERK1/2 in the CB of the rats exposed to hypoxia for 2 weeks or 3 weeks were up-regulated compared with that in group exposed to hypoxia for 0 week. The expression of p-ERK1/2 in the CB of the rats exposed to hypoxia plus rmIL-1βinjection were up-regulated compared with that in group only exposed to hypoxia. Conclusion： Both chronic hypoxia stimulation and rmIL-113 can up- regulate the expression of p-ERK1/2 in the CB ; the combination of hypoxia and rmIL-113 stimulation can significantly en- hance the expression of p-ERK1/2 in CB compared with single treatment by hypoxia or rmIL-1β.