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家蚕雄蛾触角电位并联等效电路模型的探讨
  • ISSN号:2095-1353
  • 期刊名称:《应用昆虫学报》
  • 时间:0
  • 分类:S882.2[农业科学—特种经济动物饲养;农业科学—畜牧兽医]
  • 作者机构:[1]南京林业大学,南京210037
  • 相关基金:国家自然科学基金项目(31470592); 江苏省大学生创新训练计划重点项目(201410298002Z); 南京林业大学创新基金项目(163101041)
中文摘要:

【目的】为探讨昆虫嗅觉感受机理及其化学感受行为机制提供技术基础。【方法】对家蚕Bombyx mori雄蛾触角部分切除后,测定其触角电位(EAG)变化;构建家蚕雄蛾并联等效电路模型;分析EAG与电路模拟器件间的关系。【结果】切除部分分枝后,EAG幅值与整根触角相比,除保留中部10个分枝的处理组无显著差别外,其余6组均显著减小;噪声强度有随触角分枝数量减少而增加的趋势;信噪比(SNR)有随分枝数量减少而减小的趋势;触角中部分枝数量由10个逐渐减少时,其EAG幅值随之线性减小。切除尖部主干后,EAG幅值增加,噪声强度减小,SNR增强;切除基部主干后,EAG幅值减小,噪声强度增加,SNR减弱。构建获得了基于触角主干和分枝阻抗的EAG并联等效电路模型,能较好地模拟实验测定结果。【结论】触角主干和分枝的阻抗对EAG信号输出有较大影响,EAG并联等效模型经具体化和改进后可适用于其他类型昆虫触角的EAG实验分析。

英文摘要:

[Objectives] To provide a technical basis for revealing the mechanism of olfactory reception, and chemoreceptive behavior in general, in insects. [Methods] Electroantennogram(EAG) signals of male Bombyx mori were measured after a portion of the antenna had been cut off and a parallel equivalent electrocircuit model of the resultant variations in EAG output were constructed. Correlations between actual EAG signals and the simulated circuit elements were analyzed. [Results] As long as 10 branches remained in the center of the antenna there was no significant difference in EAG amplitudes compared to an intact antenna. However, removal of additional elements of the antenna significantly decreased EAG amplitudes. Noise levels tended to increase, and SNR to decrease, as the number of antenna branches decreased. EAG amplitudes decreased in a linear fashion, as the number of branches on the center of the antenna decreased below 10. Removal of the top portion of the antenna trunk caused the EAG amplitude to increase and the noise level to decrease, thereby enhancing SNR. Removal of a basal portion of the antenna trunk caused EAG amplitude to decrease and the noise level to increase, thereby reducing SNR. A parallel equivalent electrocircuit model of EAG output, based on the impedance of the antenna trunk and branches, was constructed, that simulated the experimental results. [Conclusion] The impedance of the antenna trunk and branches have a great influence on the strength of the EAG signal. With some modifications, the parallel equivalent electrocircuit model developed in this study should be applicable to the experimental analysis of the EAG output of other insects.

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期刊信息
  • 《应用昆虫学报》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国昆虫学会 中国科学院动物研究所
  • 主编:戈峰
  • 地址:北京朝阳区北辰西路1号院5号中科院动物所
  • 邮编:100101
  • 邮箱:entom@ioz.ac.cn
  • 电话:010-64807137
  • 国际标准刊号:ISSN:2095-1353
  • 国内统一刊号:ISSN:11-6020/Q
  • 邮发代号:2-151
  • 获奖情况:
  • 96、2000年获中科院《优秀期刊三等奖》,92年获中国科协《优秀学术期刊一等奖》,2001进入“中国期刊”方阵,“双百”期刊,排名第96位
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:3170