中文摘要：

为进一步鉴定试验菌株及明确一色齿毛菌CB1（Cerena unicolor CB1）对活性染料的脱色效果，在对该菌株ITS序列克隆的基础上，进行了其对活性黑和活性红2种活性染料脱色条件的研究。结果表明，C.unicolorCBl与17个同种其他菌株的ITS序列相似性为93％～99％，说明试验菌株为一色齿毛菌。染料脱色结果表明，250mg／L的活性黑对C．unicolorCBl的脱色产生明显的抑制，而500mg／L的活性红对该菌株的脱色抑制不明显。C．unicolorCBl对活性黑和活性红的脱色最适碳源分别是果糖和葡萄糖；最适氮源分别是尿素和硝酸铵；最适Cu^2＋和Mn^2＋添加浓度都为0．1mmol／L；最适pH为5；最适接种量为直径d=8mm的新鲜菌丝7片。

英文摘要：

A study for determining the decolorizing capacity of white rot basidiomycete Cerena unicolor strain CB1 was carried out. Based on cloning of ITS sequence, factors affecting decolorization into two dyes, reactive black and reactive red, were carried out. The results of phylogenetic analysis showed that the ITS sequence of C. unicolor CB1 was 93% to 99% homologous to 17 different C. unicolor strains, indicating that strain CB1 was C. unicolor. The results of decolorization tests showed that the reactive black dye inhibited the decolorization reaction of C. unicolor CB1 evidently at the concentration of 250 mg/L, whereas reactive red had no significant inhibition to the decolorization reaction even at the concentration of 500 mg/L. The most optimal carbon source for C. unicolor CB1 to decolor reactive black and reactive red was fructose and glucose, respectively; The best nitrogen source was urea and ammonium nitrate, respectively. The optimal concentration of Cu^2＋ and Mn^2＋ both was 0. 1 mmol/L, the optimum pH was 5, and the optimum inoculum was seven fresh mycelium discs with d = 8 mm.