中文摘要：

目的探讨miR-16表达水平与金黄色葡萄球菌脓毒症严重程度的关联性,进一步探讨其潜在的临床意义。方法收集金黄色葡萄球菌脓毒症血液标本共计32例,脓毒性休克、严重脓毒症和一般脓毒症各8例,不同年龄段的健康对照24例;另外,收集革兰氏阴性菌脓毒症血液标本8例。用Trizol液裂解全血后提取microRNA,采用荧光定量PCR测定miR-16在不同组的表达水平（2-△△Ct法）,用SPSS 13.0软件分析各组之间的统计学差异。采用SPSS 13.0软件将miR-16定量值与相应CRP和PCT值进行相关性分析。结果 miR-16表达水平与金黄色葡萄球菌脓毒症严重程度呈明显的负关联,各实验组与对照组相比均有统计学差异（P〈0.001）,实验组之间也有统计学差异（P〈0.01）。miR-16表达水平与CRP和PCT值呈负相关性,相关系数分别为-0.561和-0.769。结论 miR-16表达与金黄色葡萄球菌脓毒症有明显的负关联性,提示其可能作为该菌脓毒症严重程度的标记物。

英文摘要：

We investigated correlation between the level of miR-16 expression and the severity of Staphylococcus aureus sepsis, and further explored its potentially clinical significance. Blood samples were collected from 32 patients, including each 8 cases of septic shock, severe sepsis and general sepsis, as well as 8 cases of healthy volunteers. Blood samples from 24 cases of healthy subjects with different ages were measured, and additionally 8 cases of blood samples from gram negative bacteria sepsis were also determined in current study as a control. Trizol solution for the whole blood lysis was added into blood samples, and followed by the extraction of microRNA. The expression levels of miR-16 in different groups were measured by fluorescence quantitative PCR, in which 2-Delta Ct method was used. SPSS software （version 13.0） was used to analyze the statistical differences between the groups, and further analyze the correlation between miR-16 value and the corresponding CRP and PCT values. Results showed that the expression level of miR-16 was negatively correlated with the severity of sepsis. There were statistically significant differences in experimental groups when compared with the control （P〈 0.001）, and there was also a statistically significant difference between each experimental group （P〈0.01）. We found that the expression level of miR-16 was negatively correlated with CRP and PCT, the correlation coefficients were -0.561 and -0.769 respectively, and trend analysis showed that there was a significantly negative correlation. A significantly negative correlation was found between the miR-16 expression level and severity of sepsis, suggesting that miR-16 may serve as a biomarker for the severity of Staphylococcus aureus sepsis.Staphylococcus aureus