中文摘要：

Tylenchulus semipenetrans 是在中国的柠檬的最经济地重要、普遍的线虫害虫。rDNA -- 它的 of T 的 14 张人口。从不同柠檬林子或中国冷杉木(Cunninghamia lanceolata ) 镇定的 semipenetrans 在中国的种植园被放大并且定序。种类特定的教材第一次被设计到诊断 T。semipenetrans 基于 rDNA 的序列 -- 它上面的地理人口的区域。教材是敏感的从一个单个秒阶段少年(J2 ) 或 T 的不同生活阶段的 DNA 模板放大期望的乐队尺寸(297 bp ) 。semipenetrans。没有特定的乐队从通常在柠檬林子被发现的 15 非目标线虫种类被放大。教材的特性和可靠性被 T 的 16 张额外的人口的进一步的 PCR 扩大验证。从中国的 4 个省镇定的 semipenetrans。教材成功地检测了 T 的单个 J2。在包括 a large numbers of 非目标线虫的一个整个线虫社区以内的 semipenetrans。发达诊断技术能被用于 T 的精确鉴定。semipenetrans 并且另外作为为为在中国的柠檬或中国冷杉木的线虫管理的一个决定工具。

英文摘要：

Tylenchulus semipenetrans is the most economically important and widespread nematode pest of citrus in China.rDNA-ITS of 14 populations of T.semipenetrans which were collected from different citrus groves or Chinese fir（Cunninghamia lanceolata） plantations in China were amplified and sequenced.The species-specific primers were designed for the first time to diagnosis T.semipenetrans based on the sequences of rDNA-ITS regions of geographic population above.The primers were sensitive to amplify the expected band size（297 bp） from DNA template of a single second-stage juvenile（J2） or different life stages of T.semipenetrans.No specific band was amplified from 15 non-target nematode species which were commonly found in citrus groves.Specificity and reliability of the primers were validated by further PCR amplification of 16 extra populations of T.semipenetrans collected from 4 provinces of China.The primers successfully detected a single J2 of T.semipenetrans within a whole nematode community comprising a large numbers of non-target nematode.The developed diagnostic technique can be used for accurate identification of T.semipenetrans and also as a decision tool for nematode management for citrus or Chinese fir in China.