中文摘要：

目的：探讨温针灸对类风湿性关节炎（RA）大鼠关节滑膜组织沉默信息调节因子2相关酶1（SIRT 1）表达的影响,以及对核转录因子-κB（NF-κB）的调节作用,分析温针灸改善RA的机制。方法：SD大鼠50只（雌雄各半）,随机分为正常组、模型组、抑制剂组、温针灸组、抑制剂＋温针灸组,每组10只,采用皮内注射牛Ⅱ型胶原接种诱发制备大鼠RA模型,共21d。温针灸组于造模第1天取“足三里”“肾俞”和“悬钟”温针灸治疗,刺激量以大鼠耐受为度,1次/d,共21d。抑制剂组于造模第1、7、14、21天腹腔内注射SIRT 1抑制剂尼克酰胺溶液。抑制剂＋温针灸组于造模同时给予SIRT 1抑制剂,温针灸治疗同温针灸组。所有大鼠采用排水法测肿胀关节部体积,放射免疫法检测血清炎性因子IL-1β、IL-6和IL-8含量,免疫印迹法检测膝关节滑膜组织SIRT 1和NF-κB p 65蛋白的表达。结果：与正常组相比,模型组大鼠关节肿胀明显,血清中炎性因子IL-1β、IL-6和IL-8含量较高,膝关节滑膜组织SIRT 1蛋白表达减少,NF-κB p 65蛋白表达增加（P〈0.01）。与模型组相比,温针灸组大鼠关节肿胀改善明显,炎性因子IL-1β、IL-6和IL-8含量减少,SIRT 1蛋白表达升高,NF-κB p 65蛋白表达降低（P〈0.05）。与温针灸组相比,抑制剂＋温针灸组大鼠关节肿胀改善程度较差（P〉0.05）;血清中炎性因子IL-1β、IL-6和IL-8含量较高,膝关节滑膜组织SIRT 1蛋白表达减少,NF-κB p 65蛋白表达增加（P〈0.05）。结论：温针灸能有效降低RA大鼠血清炎性因子,调节关节滑膜组织SIRT 1和NF-κB p 65表达,这可能是温针灸改善RA关节炎性损伤的机制之一。

英文摘要：

Objective To observe the effect of warm needle moxibustion （WNM） on knee-joint swelling and expression of sirtuin 1 （SIRT 1, a class Ⅲ histone/protein deacetylase） and nuclear factor KB （NF-κB） in synovial tissue in rats with rheumatoid arthritis （RA）. Methods Fifty SD rats were randomly divided into five groups： normal control, model, SIRT 1-inhibitor, WNM, and SIRT 1-inhibitor plus WNM （inhibitor＋ WNM）, n = 10 rats in each group. The RA model was established by subcutaneous injection of bovine type Ⅱ collagen at the sole, once daily for 21 days. The WNM was applied to bilateral ＂Zusanli＂ （ST 36）, ＂Shenshu＂ （BL 23） and ＂Xuanzhong＂ （GB 39） for 15 min, once daily for 21 days, beginning on the 1^st day of modeling. For rats of SIRT 1-inhibitor group and inhibitor＋ WNM group, nicotinamide solution （10 mmol ·kg^-1 · d^-1） was injected intraperitoneally on the 1^st, 7^th, 14^th and 21^th days of modeling. The swelling volume of the affected knee-joint was measured by water drainage method. The contents of IL-1β, IL-6 and IL-8 in the serum were measured by radioimmunoassay. The expression of SIRT 1 and NF-κB p 65 proteins in the synovial tissue of knee-joint were detected by immunoblotting. Results After modeling, the knee-joint volume, serum IL-1β, IL-6 and IL-8 contents, and the expression of NF-κB p 65 protein in synovial tissue of knee-joint were considerably increased relevant to the normal control group （P〈0.01）, while the expression of synovial SIRT 1 protein was significantly down-regulated （P〈0.01）. Following WNM intervention, the volume of the knee-joint, serum IL-1β, IL-6 and IL-8 contents and synovial NF-κB p 65 expression were significantly decreased relevant to the model group （P〈0.05）, and the down-regulated expression of SIRT 1 protein was notably suppressed （P〈0.05）. No significant difference was found between the WNM and inhibitor＋ WNM groups in the knee-joint swelling degree （P〉0.05）. The contents of se