中文摘要：

云南铁角蕨Asplenium yunnanense与泸山铁角蕨Asplenium lushanense的分类是一个悬而未决的问题。本文对云南铁角蕨与泸山铁角蕨及近缘类群的叶绿体rbcL基因和trnL-F、rps4-trnS基因间隔区序列进行PCR扩增和序列分析；并与已经发表的铁角蕨属植物的相应序列进行比较，发现云南铁角蕨与泸山铁角蕨的rbcL基因和trnL-F、rps4-trnS基因间隔区序列之间均未表现出差异，因此叶绿体DNA序列的证据不能将云南铁角蕨与泸山铁角蕨分开，从而从分予系统学方而证明云南铁角蕨与泸山铁角蕨是一对亲缘关系非常近的物种。

英文摘要：

The relationship between Asplenium yunnanense Franch. and Asplenium lushanense C. Chr. has long been in dispute, and here was evaluated based on the sequence analysis of chloroplast rbcL gene, trnL-F and rps4-trnS intergenic spacers. No sequence difference in the studied chloroplast DNA regions was found between Asplenium lushanense and A. yunnanense, but some nucleotide differences in the corresponding DNA fragments were found among other Asplenium species. So, A. yunnanense and A. lushanense cannot be distinguishable in terms of chloroplast DNA sequences, and the very close relationship between them is further affirmed based on the evidence from molecular data.