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中文摘要：
为了研究氯细胞（CCs）在中华鲟（Acipenser sinensis Gray,1835）幼鱼鳃上的分布状况及形态特征,通过石蜡切片-HE染色法及冰冻切片免疫荧光染色法对氯细胞进行了定位研究。研究表明：利用棋盘法,确定一抗和二抗的最佳实验浓度分别为1∶1 500和1∶500时定位其鳃上的Na＋/K＋-ATPase免疫阳性细胞（NKA-IR细胞,即氯细胞）效果较好,可用于中华鲟幼鱼鳃上氯细胞的免疫定位研究。鳃丝与鳃小片上氯细胞数量分别为（4.72±0.34）个/100μm和（0.64±0.19）个/100μm,差异极显著（P〈0.001）;鳃丝与鳃小片上氯细胞平均面积无显著性差异（P〉0.05）,分别为（187.28±18.30）μm2和（159.53±16.72）μm2;鳃丝与鳃小片上氯细胞的细胞形状因子分别为0.72±0.33和0.68±0.46,差异不显著（P〉0.05）。由此可见,中华鲟幼鱼鳃上氯细胞呈卵圆形,主要分布在鳃丝上,尤其是鳃丝及鳃小片基部区域,少量分布在鳃小片上,鳃丝与鳃小片上氯细胞大小基本一致。
英文摘要：
In order to study the distribution pattern of chloride cells （CCs） on the gills of the juvenile Acipenser sinensis, immune localization of CCs was studied by using the methods of HE and immunofluoreseence staining in this paper. The results indicated that the optimal dilution of the first antibody α5 and the fluorescent second antibody Alexa 488 donkey-anti-mouse was 1 ： 1 500 and 1 ： 500, respectively, which had good effect on the location of Na ＋ -K ＋ -ATPase immunoreactive cells （NKA-IR cells, i. e. , CCs） and could be used to locate the CCs on the gills of juvenile A. sinensis. The number of the CCs on gill filaments （4.72 ＋ 0.34 ind/100 Ixm） was significantly （P 〈 0. 001） greater than that on gill lamella （0.64 ±0.19 ind,/100μm）. The mean area of the CCs had no significant difference （P 〉0.05） between gill filaments （ 187.28 ± 18.30 μm2） and lamella （ 159.53 ± 16.72 μm2）. The shape factor of the CCs in the gill filaments and gill lamella was 0.72 ± 0.33 and 0.68 ± 0.46, respectively, which showed no significant difference （P 〉 0.05 ） between them. Thus, the CCs of juvenile A. sinensis were oval and mostly found on the gill filament, especially at the base, with a small amount on gill lamella. The CCs on the gill filament and lamella were of the same size.
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