中文摘要：

为了利用分子标记技术研究湖北海棠的起源、系统发育和分类问题，本研究对湖北海棠及其近缘种叶DNA的CTAB提取法进行改良，并对SSR体系优化。结果表明，将EDTA用量从20mmol／L提高到80mmol／L，加大PVP和RNA酶的用量，有助于提高从湖北海棠叶中提取的DNA质量；利用反复实验优化的SSR体系，可从40对引物中筛选出18对多态性好、条带清晰的湖北海棠SSR引物。本研究筛选出的多态引物，将为探讨湖北海棠的起源、进化和遗传变异等提供基础资料。

英文摘要：

In order to study the origin, systematic development and the taxonomic problems ofM. hupehensis, the CTAB extraction methods for DNA of M. hupehensis and several related species were improved in this paper and the SSR system has been optimized. The results showed that it is helpful to improve the DNA quality which was extracted from leaves of M. hupehensis by increasing the concentration of EDTA from 20 mmol/L to 80 mmol/L, the amount of PVP and RNA enzyme. Through the optimal SSR system, 18 primers with high polymorphism were selected from 40 primers. These polymorphic primers will provide the basic information for the origin, evolution, and genetic variation ofM. hupehensis.