中文摘要：

观察黄芪超滤物（UEMAM）对H22腹水瘤小鼠重离子放疗的增敏作用,并探讨其相关的机制。小鼠腹腔内注射H22腹水瘤细胞悬液0.2 mL制备腹水瘤小鼠模型。成模小鼠随机分为4组,分别给予生理盐水（对照组）、UEMAM（中药组）、6Gy~（12）C~（6＋）离子束外照射（放疗组）及UEMAM联合6Gy~（12）C~（6＋）离子束外照射（中药＋放疗组）处理。每天测量小鼠的体重、腹围,并观察其日常生活状态;统计各组小鼠的生存期,流式细胞仪检测细胞的凋亡率及周期分布;Western blot检测p53、Bax、Bcl-2及cleaved Caspase-3的表达;单细胞凝胶电泳技术观察H22细胞DNA的损伤情况。实验结果表明治疗组（中药组、放疗组及中药加放疗组）小鼠平均生存时间较对照组明显延长,体重与腹围较对照组明显减小;与对照组相比,治疗干预后促进了H22细胞凋亡、周期阻滞及DNA损伤;中药加放疗组的平均生存时间较放疗组明显延长,体重与腹围较放疗组明显减小;此外,中药加放疗组还促进了H22细胞凋亡、周期阻滞及DNA损伤;蛋白表达结果分析治疗组（中药组、放疗组及中药加放疗组）明显激活了p53凋亡信号通路。通过该实验发现UEMAM对H22腹水瘤小鼠具有放射增敏作用,其增敏作用机制与激活p53介导的细胞凋亡信号途径有关。

英文摘要：

This study was designed to investigate the impact of ultra-filtration extract mixture from Astragalus mongholicus （UEMAM） on radiosensitivity of H22 ascitic tumor in mice to 12C6＋ ions radiation. The H22 ascitic tumor model was established in mice by intraperitoneal injection of 0.2 mL H22 ascitic cells. The animals were subsequently divided into 4 groups randomly, treated with normal saline, UEMAM, heavy ion beam radiotherapy and UEMAM plus heavy ion beam radiotherapy, respectively. The body weights, abdomen circumference of the mice were measured and the mouse behavior was monitored every day; survival time was recorded to evaluate life extension effect; flow cytometry technique was used to detect H22 cell apoptosis and cell cycle; protein levels of p53, Bax, Bcl-2 and cleaved Caspase-3 were analyzed by Western blot; the single cell gel electrophoresis was used to detect the level of deoxyribonucleic acla oamage tLP~s,~ uam~t~）. results suggest that UEMAM significantly increased survival time, and decreased body weights and abdomen circumference over the saline control group. The treatment increased cell apoptosis, cycle arrest and DNA damage compared to the saline control group. UEMAM significantly enhanced the therapeutic effect of heavy ion beam radiation in survival time, and decreased body weights and abdomen circumference in the tumor-baring mice. The combination increased cell apoptosis, cycle arrest and DNA damage compared to the radiotherapy group. The results of Western blot suggest that the treatment significantly enhanced p53-induced apoptotic signals. The experiment discovered that UEMAM could improve radiosensitivity of H22 ascitic tumor through activation ofp53-rnediated apoptotic signal pathway.