中文摘要：

目的：评估胚胎植入前遗传学诊断（PGD）出生小鼠对高温环境的应激能力。方法：构建小鼠模型，分别为实验组PGD小鼠和对照组Normal小鼠。在5周龄时，将两组小鼠置于45℃高温环境，分析比较它们对热应激的反应调节能力。结果：45℃高温应激下，PGD小鼠平均耐受至死亡时间为59．31min，显著低于Normal组平均死亡时间79.63min（P〈0．001）。在45℃30min应激下，两组小鼠应激激素水平相似：中枢应激器官——蓝斑和下丘脑室旁核的组织结构及神经元活性在两组小鼠间无明显差异；外周应激器官——肾上腺皮质和髓质的组织结构，以及皮质合成分泌皮质酮的功能、髓质合成分泌肾上腺素的能力在两组小鼠间均无差异。结论：PGD小鼠对45℃高温环境的反应调节能力与Normal小鼠存在差异，这一异常可能不是由蓝斑、下丘脑以及肾上腺的功能差异直接造成的，而与它们所分泌激素的进一步作用有关。需要对应激激素的靶器官进行全面的结构、功能评估。

英文摘要：

Objective：To evaluate the impact of preimplantation genetic diagnosis procedures on the heat stress response in the offspring using mouse model. Methods：We use the mouse models,which divided into PGD group and Normal group. At 5 weeks of age, evaluate the stress system of PGD offspring under 45℃ heat stress. Results： Under heat stress, the mean death time is 59.31 min and 79.63 min in PGD and Normal group, respectively（P 〈 0.001）. There is no significant difference between the two groups of mice under the stress, such as serum hormones, structure and neuronal activity of locus coeruleus and hypothalamus. Similarly, there is no significant difference of adrenal structure between two groups, and the level of adrenaline, noradrenaline and corticosterone is the same trend. Conclusion：The different response ability between two groups under heat stress is not related to the function of locus coeruleus.hvnothalamus and adrenal. It mav he related to the further function of their secretioned hormones.