欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
Tb作为镧系元素中少数几个有荧光性质的稀士离子,在荧光方面有很多重要的应用。如Tb被广泛用于与蛋白质钙位点结合的荧光探针,同时也可根据Tb的荧光特性,用荧光方法定量分析Tb-有机配体配合物。本研究发现HEPES（常用的pH缓冲剂）可显著增强Tb在585nm的特征荧光发射峰,同时微弱的增强Tb在490nm和549nm的特征荧光发射峰。一直以来,经常使用Tb在549nm处的特征荧光发射峰定量研究Tb化合物的浓度;然而,本研究却发现在进行此类实验时需要慎重选择实验条件,因为Tb荧光的定量分析结果会随缓冲试剂浓度或者含量的变化而改变。另一方面,Tb 585nm处的特征荧光发射峰的荧光强度与HEPES和Tb离子的浓度都有一定的依赖关系,因此Tb 585nm特征荧光发射峰也有被用于荧光定量分析研究的潜能。
英文摘要：
Terbium （Tb） has been extensively used as a fluorescence probe for the identification of calcium-binding sites in proteins and for fluorometric analysis of organic ligands. In the current study, we reported that HEPES, a commonly used pH buffer reagent, significantly enhanced the characteristic emission of Tb at 585 nm. The maximum emission of Tb at 490 nm and 549 um were also enhanced by HEPES to a less extent. Thus, cautions should be taken when quantitative analysis is performed based on the fluorescence emission of Tb at 549 nm, since the emission may vary due to the buffer reagents. Additionally, the fluorescence intensity at 585 um was proportional to the concentration of both HEPES and terbium ions, which might be utilized to develop new fluorometric analytical methods.
同期刊论文项目
同项目期刊论文
Endothelial cell and macrophage regulation of vascular smooth muscle cell calcification modulated by
Metal-amyloid-beta peptide interactions: a preliminary investigation of molecular mechanisms for Alz
Gadolinium triggers unfolded protein responses (UPRs) in primary cultured rat cortical astrocytes vi
Enhancement of lanthanum (iii) on sodium currents in acutely isolated hippocampal CA1 neurons of rat
Duality of effect of La3+ on mitochondrial permeability transition pore depending on the concentrati
Brain-derived neurotrophic factor protects neurons from GdCl 3-induced impairment in neuron-astrocyt
Impaired mitochondrial function and oxidative stress in rat cortical neurons: Implications for gadol
Gadolinium-containing bioparticles as an active entity to promote cell cycle progression in mouse em
Lanthanum Chloride promoted proliferation with enhanced S-phase entry and inhibited K currents of NI
Studies on the acute biochemical effects of Nd(NO3)(3) by nuclear magnetic resonance-based metabonom
Lanthanum chloride suppresses hydrogen peroxide-enhanced calcification in rat calcifying vascular ce
Gadolinium-promoted cell cycle progression with enhanced S-phase entry via activation of both ERK an
A proteomic investigation into the human cervical cancer cell line HeLa treated with dicitratoytterb
Cytoskeleton reorganization and FAK phosphorylation are involved in lanthanum (III)-promoted prolife
Vanadium compounds discriminate hepatoma and normal hepatic cells by differential regulation of reac
Role of reactive oxygen species in the antiproliferative effects of metavanadate on human prostate c
Reactive-oxygen-species-mediated Cdc25C degradation results in differential antiproliferative activi
期刊信息
位置：