中文摘要：

芪类物质是葡萄材料中一大类具有特殊药理活性的天然产物，实际操作中由于葡萄叶片含量过低而导致利用率不足。已有报道UV、氯化铝、过氧化氢等非生物诱导剂能够引起一些植物材料芪类物质的积累的同时又能引起材料氧化胁迫。为了研究这3种诱导剂在葡萄离体叶片上的诱导特性及与氧化胁迫的关系，本文首次使用上述3种诱导剂对红地球葡萄离体叶片中4种主要芪类物质含量变化进行分析，并研究过氧化氢酶（CAT）及N-乙酰．L-半胱氨（N-acetyl-L—cysteine）预处理后芪类物质变化规律。诱导剂处理后，自藜芦醇、白藜芦醇苷、紫檀芪、ε-viniferin等芪类物质都有不同程度的升高，其中紫外照射处理10min，暗室放置24h对自藜芦醇的诱导效果最为明显，鲜重达到200μg／g。加完抑制剂CAT和NAC后，芪类物质变化不规律，其中H2O2诱导加抑制剂CAT对白藜芦醇的抑制效果最佳，含量从113μg／g降低到16．5μg／g。结果说明，3种诱导剂对红地球葡萄离体叶片芪类物质类别及其含量具有特殊的影响作用，且此与材料内部氧胁迫水平存在一定联系。

英文摘要：

The content of the stilbenes were lower in normal grape leaves,so it is difficult as the use of medicinal ex- tract. The abiotic inducers （H202, UV,AIC13 ） previously reported to elevated the contents of stilbenes in various materials with oxidative stress simultaneously. In this study, aims to study the relationships between 4 stilbenes accumulation and oxi- dative stress, grape leaves were induced by the abiotic inducers and then processed by the corresponding inhibitors （N-ace- tyl-L-cysteine）. The content changes of resveratrol, polydatin, pterostilbene and ε-viniferin were analyzed by HPLC. The re- suits showed that, after the treatment of the inducers, the stilbene had different degrees, of which the effect of UV-induced is the most obvious,the maximum content of resveratrol 200μg/g in treated leaves. Alter the inhibitors（ CAT, NAC）weread- ded. the content changes of the stilbenes were not in the law, inhibitors of H2 02-induced and CAT on the inhibitory effect of resveratrol is best,the content of resveratroJfrom ll3μg/g reduced to 16.5μg/g. The result showed that,Three kinds of in- ducers have the special influence function to the detached grape leaves （Vitis vinifera cv. Red Globe）of category and the content, also material interior oxygen coercion level existence certain relation.