中文摘要：

纳米TiO2光催化氧化-免疫-电生孔复合技术能够在低的纳米TiO2浓度条件下（3．12μg·mL^-1）高效选择性地杀伤LoVo肠癌细胞。在光强为4mW·cm^-2的紫外光（波长253．7nm）照射下，30min内可全部杀死癌细胞。利用共聚焦荧光显微镜、透射电镜（TEM）和单细胞凝胶电泳的方法研究了其作用过程。结果表明，经抗体修饰的纳米TiO2微粒能自动吸附在癌细胞的细胞膜上，在电脉冲作用下纳米TiO2可进入细胞内部，并主要集中在细胞核区域。在紫外光的照射下，基于纳米TiO2的光催化氧化作用，造成细胞内一些细胞器、核膜和核中DNA的损伤，使细胞坏死。由于是在细胞内部产生光催化氧化作用，显著提高了杀伤LoVo肠癌细胞的能力。

英文摘要：

The nano TiO2 photocatalytic oxidation method combined with immunity and electroporation techniques performed high efficiency for photokilling LoVo cancer cells even at very low concentration （3.12μg·mL^-1）. In this method, the LoVo cells could be killed completely within 30 rain under the irradiation of UV light （wavelength 253.7 nm, 4 mW·cm^-2）. The mechanism of this process was investigated using confocal florescent microscopy, TEM, and comet assay methods. The results demonstrated that the antibody modified nano TiO2 particles could adsorb on the cell membrane, after electroporation it could enter the inside of LoVo cells and located around the karyon. Under the irradiation of UV light, the cell＇s structure, organelle, and DNA were destroyed seriously, which caused the necroses of LoVo cells. Because the photocatalysis effect of nano TiO2 took place inside of cells, so the photokilling efficiency enhanced strongly.