中文摘要：

建立了金纳米棒一鲁米诺一硝酸银化学发光新体系，发现金纳米棒比金纳米球具有更强的催化作用．利用化学发光光谱、X射线光电子能谱（XPS）、能量散射X射线谱（EDX）对该体系进行了表征，考察了各种反应条件、纳米的形貌及保护试剂对化学发光反应的影响，提出了化学发光反应的机理．基于此新体系，将金纳米棒用于免疫标记，建立了一种测定人免疫球蛋白IgG的微孔板化学发光免疫分析新方法，线性范围为25～1000μg／L，检测限为15．3μg／L．

英文摘要：

A new chemiluminescence （CL） system gold nanorods-luminol-AgNOa was built and gold nanorods were found to exhibit higher catalytic activity towards luminol CL than gold nanospheres. CL spectra, X-ray photoelectron spectroscopy （XPS）, energy dispersive X-ray spectroscopy （EDX） were used to characterize this system. The effects of various reaction conditions, the morphology and protection reagents of nanoparticles on the CL reaction were also investigated. The mechanism of the CL reaction was proposed. Finally a novel microplate-compatible chemiluminescence immunoassay based on this new CL system was developed for the determination of human immunoglobulin G （IgG） with a linear range from 25to 1 000 μg/L and a detection limit of 15.3μg/L.