中文摘要：

目的研究人免疫缺陷病毒（HIV-1）对不同黏膜上皮细胞系的感染能力。方法用实验室株HIV-1 SF33和2株原代HIV-1（02010561，02010141）分别感染Caco-2、T-84和HeLa3株黏膜上皮细胞和MT-4细胞。接种病毒后间隔3～4d采集培养上清检测P24并用实时定量RT-PCR检测病毒载量；采集细胞提取DNA并用PCR法检测感染细胞中病毒DNA和整合入细胞基因组内的病毒DNA。结果所用3株病毒都可以产毒性地感染阳性对照细胞MT-4，对整合病毒DNA的PCR检测发现它们均能够整合到MT-4细胞基因组内；实验室适应株HIV-1 SF33虽然能够感染所有3株上皮细胞，但它不能整合入Caco-2细胞的基因组中；虽然2株原代分离病毒均能感染T-84细胞，但只有HIV-1 02020141能够整合入T-84细胞的基因组中，原代分离病毒HIV-1 02010561能够感染HeLa细胞，但不能整合到其基因组中。结论虽然HIV-1的实验室毒株和原代分离毒株都可能感染黏膜上皮细胞，但它们在黏膜上皮细胞中建立稳定产毒性感染（感染并产生病毒）的能力因细胞和毒株不同而异。

英文摘要：

Objective To compare the infectivity between laboratory adapted human immunodefi- ciency virus（HIV-1） and primary HIV-1 isolates for different mucosal epithelial cell lines. Methods Mu- cosal epithelial cells Caco-2, T-84, HeLa and lymphocyte MT-4 were infected with laboratory adapted HIV-1 SF33 and 2 primary HIV-1 isolates （02010561, 02010141）. Culture supernatant and cells were collected respectively on 3-4 days interval after virus inoculation. The former was tested for HIV-1 antigen P24 level and viral load, and the latter was tested for total viral DNA and integrated viral DNA. Results All 3 virus strains could infect MT-4 cells and integrate into their genome. Only HIV-1 SF33 could infect Caco-2 cells but could not integrate into their genomic DNA. Both HIV-1 SF33 and 02010561 infected HeLa cells but only integration of HIV-1 SF33 was detected. All the 3 HIV-1 strains infected T-84 cells but only the integra- tion of HIV-1 SF33 and 02010141 was observed. Conclusion Although laboratory adapted and primary HIV-1 strains are able to infect human mucosal epithelial cell lines, transient or productive infection established in different mucosal epithelial cells is dependent on the character of cells and virus strains.