中文摘要：

目的探讨靛玉红对免疫性血小板减少性紫癜（ITP）小鼠模型的治疗作用。方法 Wistar大鼠血小板免疫CBA小鼠20只,建立ITP动物模型,随机分为两组,实验组给予靛玉红腹腔注射,对照组给予PBS腹腔注射,在建模第4周分离小鼠脾细胞,MTT检测脾细胞增殖,流式细胞仪检测小鼠脾中淋巴细胞表型（CD4、CD8、CD25、Foxp3）,并以MACS分离CD4＋CD25＋Foxp3＋调节性T细胞（Treg）进行混合淋巴细胞培养（MLR）,评价调节性T细胞的抑制功能。结果靛玉红通过抑制ITP小鼠脾细胞增殖,减少CD4细胞的数量,提高CD4＋CD25＋Foxp3＋调节性T细胞的相对比例,有效改善ITP的血小板减少程度,而MLR显示靛玉红并不抑制Treg的抑制功能。结论靛玉红可通过影响调节性T细胞诱导ITP免疫耐受。

英文摘要：

Objective To study the mechanism of Indirubin on the immune thrombocytopenic purpura（ITP）mice model.Methods The ITP animal model was established with CBA mice which received Wastar rat platelets.At the fourth week after the Indirubin treatment,spleen cells isolated from ITP mice was detected with MTT for proliferation,while cell levels and phenotypes were observed under FCM.The immunoregulatory function of CD4＋CD25＋Treg cells isolated by MACS was evaluated with MLR.Result Proliferation of spleen cells in the ITP mice treated with indirubin was significantly inhibited.The ratios of CD4＋CD25＋Treg cells or CD4＋CD25＋Foxp3＋ Treg cells to CD4＋T cells in spleens（P〈0.01）enhanced by Indirubin were identified.Also,splenic CD4＋CD25＋Foxp3＋ Treg cells treated with Indirubin showed immunosuppressive ability on the immune response of T effector cells to alloantigens or mitogen as efficiently as the control,CD4＋CD25＋Treg cells,in vitro.Conclusion The present study indicates that Indirubin can induce immune tolerance in ITP mice by exerting effects on the number and relative proportions of CD4＋CD25＋Treg cells.