中文摘要：

经过分子筛层析和离子交换层析，我们从竹叶青（Trimeresurus stejnegeri）蛇毒中纯化得到了竹叶青蛇毒L-氨基酸氧化酶（TSV-LAO）。实验表明，TSV-LAO是一种糖蛋白，其分子由非共价连接的两个相同的亚基组成。SDS-聚丙烯酰氨凝胶电泳一个亚基的表观分子量为58kDa。TSV-LAO酶比活力为1100U／mg，其分子脱掉糖基化成分后不影响酶活力。TSV-LAO在浓度为1．0μg/mL以上时可以诱导C8166细胞凋亡。ISV-LAO对实验病原微生物具有选择性抗生作用并具有明显的量效关系，对白色念珠菌（ATCC2002）、金黄色葡萄球菌（ATCC2592）和短小芽孢杆菌（CMCCB11207）的最小抑菌浓度分别是0．3，0．4和1．0μg／mL，即使在最高实验浓度10μg/mL，TSV-LAO对其它实验菌株也未显示抑菌作用。

英文摘要：

A novel L-amino acid oxidase from venom of Chinese snake Trimeresurys stejneger, named TSV-LAO,was purified to homogeneity by two steps of isolation including Sephadex G-100 gel fihration and Q-Sepharose ion-exchange chromatography. TSV-LAO is a glycoprotein and composed of two non-covalent identical subunits with a molecular weight of 58 kDa by SDS-polyacrylamide gel electrophoresis. The specific enzymatic activity of TSV-LAO purified preparations was 1100 U/mg. The enzymatic activity was not lost after deglycosylation. TSV-LAO above 1.0 μg/mL induces apoptosis on C8166 cells dose-dependently. TSV-LAO had antimicrobial activity in a dose-dependent and strain-selective manner. Minimal protein concentration required for total inhibition of the growth of sensitive detected strains of microbe in liquid medium （MIC） was 0.3,0.4 and 1.0 （μ/ mL） on C. albicans （ATCC2002）, S. aureus （ATCC2592） and B. megaterium （CMCCBll207） separately.