中文摘要：

传统二维细胞培养具有易操作、费用低、可大量应用的优点,在细胞培养领域广泛使用。但是由于细胞呈平面生长,无法构建出一个立体的微环境,且缺乏各种细胞因子的作用,因而与体内细胞状态存在差距;而三维细胞培养可以在细胞基因表达、基质分泌及细胞功能活动等方面更好地模拟体内细胞状态。该文着重论述三维细胞培养技术在肿瘤研究中的应用,包括构建肿瘤微环境、观察肿瘤生物学行为、肿瘤血管生成、研究肿瘤耐药性等,为肿瘤研究工作者提供参考。

英文摘要：

Traditional 2D cell culture technology is widely used in cell culture field,but it lacks the formation of 3D matrix scaffold and lacks specific cytokines in vivo.Cells in 3D cell culture system are similar to cells in vivo for gene expression and cell activity.The article focuses on the application of three-dimensional cell culture technology in cancer research,including the construction of tumor microenvironment,tumor biological behavior,tumor angiogenesis,drug resistance and so on and provides a reference for cancer research workers.