欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
研究了木质素桃红与蛋白质结合的共振光散射光谱. 实验结果表明, 在pH 2.56的酸性介质中, 木质素桃红与蛋白质发生静电作用产生以282.0、 346.0、 420.0 nm以及570.0 nm为特征峰的共振光散射（RLS）增强光谱. 在570.0 nm波长光激发下, 蛋白质的质量浓度与增强共振光散射强度ΔIRLS呈线性关系, 对BSA和HSA的检出限分别为39.0和22.3 ng/mL. 方法已用于尿样中总蛋白质分析.
英文摘要：
This contribution is about the interaction between Lignin Pink and proteins based on the measurements of the resonance light scattering （RLS） signals. The results indicate that the static interaction of Lignin Pink （LP） with proteins occurs and yields greatly enhanced RLS signals at 282.0, 346.0, and 420.0 and 570.0 nm at pH 2.56. It was found that the enhanced RLS intensity （△IRLS） at 570.0 nm is proportional to the concentration of proteins. The limits of determination （3δ） are 39.0 ng/mL for BSA and 22.3 ng/mL for HSA respectively. The results of determinations for urine samples are in agreement with the desired values.
同期刊论文项目
同项目期刊论文
Tryptophan-contained peptide-functional nanomaterials as general spectrofluorometric reagents for en
Adsorption of penicillin–berberine ion associates at a water/tetrachloromethane interface and determ
Selective (poly T) Recognition of Thymidine Homopolymer Oligonucleotide with Cobalt(II)/4-[(5- Chlor
A quantitative method for determination of Co(II) based on the inner filter effect of reagents on th
Immunoassay by detecting enhanced resonance light scattering signals of immunocomplexes using a comm
Spectral assignments to the light emissions of fluorescent organic small molecules in aqueous medium
A coupled reagent of o-phthalaldehyde and sulfanilic acid for protein detection based on the measure
A backscattering light detection assembly for sensitive determination of analyte concentrated at the
A wide dynamic range detection of biopolymer medicines with resonance light scattering and absorptio
Determination of heparin using azure B by flow Injection analysis-resonance light scattering coupled
Label-free detection of sequence-specific DNA with multiwalled carbon nanotubes and their light scat
Conformational change detection of DNA with the fluorogenic reagent of o-phthalaldehyde- (-mercaptoe
A one-step label-free optical genosensing system for sequence-specific DNA related to the human immu
Hybridization detection of DNA by measuring organic small molecules magnified resonance lights catte
A dual-wavelength resonance light scattering ratiometry of biopolymer by its electrostatic interacti
A one-pot green method for one-dimensional assembly of gold
nanoparticles with a novel chitosan-ninh
Light-scattering signals from nanoparticles in biochemical assay, pharmaceutical analysis and biolog
A label-free visual immunoassay on solid-support with silver nanoparticles as plasmon resonance scat
A resonance light scattering ratiometry applied for binding study of organic small molecules with bi
Directly light scattering imaging of the aggregations of biopolymer bound chromic hydrolytic oligome
A novel optical immunosensing system based on measuring surface enhanced light scattering signals of
Spectral studies on the interaction of amino black 10B with DNA in the presence of cetyltrimethylamm
On the factors affecting the enhanced resonance light scattering signals of the interactions between
Selectively light scattering spectrometric detection of copper (II) based on a new synthesized oxami
Pharmacokinetic detection of penicillin excreted in urine using a totally internally reflected reson
位置：