我们测量了 dimethylsulfide (数据管理系统) 的集中,丙烯酸的酸(AA ) ,和 dimethylsulfoniopropionate (DMSP ) 在三 microalgae 的生长期间:Prorocentrum micans, Gephyrocapsa 大洋洲,和 Platymonas subcordiformis。在文化媒介的 DMSP, AA,和数据管理系统集中在海藻的生长阶段之中显著地变化了,与在晚静止的生长阶段或衰老的阶段的最高的集中。在静止生长阶段,平均 DMSP 集中每在 P 的房间。micans (0.066 5 pmol/cell ) 在 G 是乘那的 1.3。大洋洲(0.049 5 pmol/cell ) 并且 20.2 在 P 预定那。subcordiformis (0.003 29 pmol/cell ) 。AA 的平均集中是 0.044 6,在 P 的 0.026 9,和 0.003 05 pmol/cell。micans, G。大洋洲,和 P。subcordiformi s,分别地比数据管理系统的集中高(0.272, 0.497,和 0.086 2 fmol/cell,分别地) 。在房间之间有重要积极关联密度和 AA, DMSP,和数据管理系统集中。在三水藻的 DMS/AA 和 AA/(DMSP+AA ) 的比率在生长周期上显著地不同。在所有三 microalgae, DMS/AA 比率在生长时期期间是不到 25% ,建议酶的劈开小径,产生数据管理系统,不是主要 DMSP 降级小径。在 DMS/AA 比率的变化显示在老朽期间有在早生长时期和更低的率的 DMSP 的酶的故障的更高的率。在所有三 microalgae, AA/(DMSP+AA ) 比率(DMSP 的降级比率) 在指数的生长阶段期间减少了,然后增加。在这些比率的变化能近似在海藻的生长的不同阶段显示 DMSP 的劈开机制。
We measured the concentrations of dimethylsulfide (DMS), acrylic acid (AA), and dimethylsulfoniopropionate (DMSP) during growth of three microalgae: Prorocentrum micans, Gephyrocapsa oceanica, and Platymonas subcordiformis. The DMSP, AA, and DMS concentrations in culture media varied significantly among algal growth stages, with the highest concentrations in the late stationary growth stage or the senescent stage. In the stationary growth stage, the average DMSP concentration per cell in P. micans (0.066 5 pmol/cell) was 1.3 times that in G. oceanica (0.049 5 pmol/ cell) and 20.2 times that in P. subcordiformis (0.003 29 pmol/cell). The average concentrations of AA were 0.044 6, 0.026 9, and 0.003 05 pmol/cell in P. micans, G. oceanica, and P. subcordiformis, respectively, higher than the concentrations of DMS (0.272, 0.497, and 0.086 2 fmol/cell, respectively). There were significant positive correlations between cell density and AA, DMSP, and DMS concentrations. The ratios of DMS/AA and AA/(DMSP+AA) in the three algae differed significantly over the growth cycle. In all three microalgae, the DMS/AA ratios were less than 25% during the growth period, suggesting that the enzymatic cleavage pathway, which generates DMS, was not the main DMSP degradation pathway. The changes in the DMS/AA ratio indicated that there was a higher rate of enzymatic breakdown of DMSP in the early growth period and a lower rate during senescence. In all three microalgae, the AA/(DMSP+AA) ratio (degradation ratio of DMSP) decreased during the exponential growth phase, and then increased. The variations in these ratios can approximately indicate the cleavage mechanism of DMSP at different stages of algal growth.