为明确小麦品种辽春10对叶锈菌小种PHT抗病的遗传基础,利用感病小麦材料871与抗病小麦材料辽春10构建的F2:3群体,对其进行成株期抗病性鉴定和遗传分析。结果表明,辽春10中含有1个显性抗叶锈病基因,暂命名为LrLC10。利用BSA法和比较基因组学策略对该抗病基因进行分子标记分析,将LrLC10定位于小麦2BS染色体上。共构建了含有8个分子标记的LrLC10基因的连锁图,其中:CAUT253位于LrLC10的远着丝粒侧,距离为0.1cM;CAUT163和CAUT131与LrLC10共分离;CAUT239位于LrLC10的近着丝粒侧,遗传距离为0.5cM。
Common wheat ( Triticum aestivum) cultivar Liaochun10 is resistant to leaf rust(Puccinia triticina)race PHT. In order to identify the genetic basis of resistance, resistance test and genetic analysis are conducted on adult plants of F2:3 populations derived from the cross between susceptible parent 87-1 and resistant cultivar Liaochun10. The results show that LiaochunlO carries a single dominant resistance gene, which is effective at adult stage, temporally designated LrLCIO. Using bulked segregant analysis and comparative genomJcs analysis,genetic map of LrLCIO was established with eight markers. Among these markers, CAUT253 were distal to LrLCIO with a genetic distance of 0.1 cM; CAUT163 and CAUT131 were co-segregated segregated with resistant gene; CAUT239 were proximal to LrLC10 ,the genetic distance is 0.5 cM.