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Isolation and Characterization of Nine Microsatellite Markers for Red-backed Ratsnake,Elaphe rufodorsata
  • 时间:0
  • 分类:Q[生物学]
  • 作者机构:Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, Jiangsu, China
  • 相关基金:This study was carried out in compliance with the current laws of China, and was supported by grants from Natural Science Foundation of China (31200283, 31272294 and 31270571), the Priority Academic Program Development of Jiangsu Higher Education Institutions, Teaching Reform Research of Nanjing Normal University (2014-61) and Postgraduate Research Innovation Projects (KYLX_0716) of Nanjing Normal University. The authors would like to thank Yan- Qing Wu for help in sample collection and experimental procedures during the research.
中文摘要:

The red-backed ratsnake(Elaphe rufodorsata) is widely distributed in East Asia, especially China. This species is a common snake in plain river network region. In the past several decades, E. rufodorsata has dramatically declined due to the effect of human activities and over hunting for traditional Chinese medicine. We developed nine species-specific microsatellite loci in 190 individuals collected from Huzhou, Zhejiang province in China. These markers revealed a high degree of genetic diversity(13–41 alleles per locus) and heterozygosity(H O ranged from 0.266 to 0.941, and H E ranged from 0.851 to 0.937). No locus exhibited significant deviations from Hardy–Weinberg equilibrium. There was no evidence of linkage disequilibrium among pairs of loci. These microsatellite markers were described in our study will be valuable tools for the long term management and population-level studies(e.g. the population structure, genetic diversity and variation, individual paternity and evolutionary history) of the species.

英文摘要:

The red-backed ratsnake(Elaphe rufodorsata) is widely distributed in East Asia, especially China. This species is a common snake in plain river network region. In the past several decades, E. rufodorsata has dramatically declined due to the effect of human activities and over hunting for traditional Chinese medicine. We developed nine species-specific microsatellite loci in 190 individuals collected from Huzhou, Zhejiang province in China. These markers revealed a high degree of genetic diversity(13–41 alleles per locus) and heterozygosity(H O ranged from 0.266 to 0.941, and H E ranged from 0.851 to 0.937). No locus exhibited significant deviations from Hardy–Weinberg equilibrium. There was no evidence of linkage disequilibrium among pairs of loci. These microsatellite markers were described in our study will be valuable tools for the long term management and population-level studies(e.g. the population structure, genetic diversity and variation, individual paternity and evolutionary history) of the species.

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