中文摘要：

目的观察Y盒结合蛋白-1（YB-1）小干扰RNA（siRNA）对肺腺癌A549细胞株表皮生长因子受体（EGFR）的影响。方法针对YB-1mRNA设计特异性siRNA及ControlsiRNA序列转染至A549细胞株，采用逆转录-聚合酶链反应（RT-PCR）和Westernblot检测空白组、单纯Lipofectamine2000处理组、ControlsiRNA组及YB-1siRNA组细胞中YB-1和EGFR在mRNA和蛋白水平的表达；通过噻唑蓝（MTT）、侵袭实验观察各组细胞的生物学行为变化。结果RT-PCR和Westernblot结果显示实验组YB-1与EGFR的mRNA和蛋白表达降低（分别为0．3820±0．0094、0．4690±0．0032和0．1820±0．0073、0．4210±0．0049），与对照组比较差异有统计学意义（P〈0．05）。MTT检测转染72h后YB-1 siRNA组吸光度值为1．34±0．11，同时转染48h后YB-1siRNA组侵袭能力较对照组明显降低（P〈0．05）。结论YB-1siRNA能抑制肺腺癌A549细胞株中EGFR的表达，影响肿瘤增殖和侵袭能力。

英文摘要：

Objective To investigate the expression of epidermal growth factor receptor （EGFR） using the small interfering RNA （siRNA） targeting Y box-binding protein-1 （YB-1） gene in lung adeno- carcinoma cell line A549. Methods Specific siRNA and a negative control sequence in accordance with YB-1 mRNA from GeneBank were designed and then transfected into A549 cells separately. In blank con- trol group, Lipofectamine 2000 treatment group, negative control siRNA group and YB-1 siRNA group, re- verse transcriptase-polymerase chain reaction （RT-PCR） and Western blotting were applied to detect the changes of YB-1 and EGFR mRNA and protein expression. Cell proliferation was examined by using MTT assay. The ability of invasion was tested by using cell invasion assay. Results RT-PCR results showed that the expression of YB-1 and EGFR mRNA （0. 3820 ±0. 0094 and 0. 4690 ±0. 0032） in experimental group was decreased significantly as compared with other groups （P 〈 0. 01 for all） , and Western blotting results also showed the expression of YB-1 and EGFR proteins （ 0. 3820 ± 0. 0094 and 0. 4690 ± 0. 0032 ） in ex- perimental group was significantly lower than other groups （ P 〈 0. 05 for all）. After transfection the prolif- eration and invasive ability of A549 cells were significantly reduced in experimental group as compared with other control groups. Conclusion Silencing the expression of YB-1 gene by using siRNA can inhibit the expression of EGFR in lung adenocareinoma A549 cell line and thus adjust proliferation and invasion of tumor.