中文摘要：

在小猪的胃肠的道(GIT ) 的 Microbiota 能在断奶转变期间经历能导致生长减小和断奶的小猪的腹泻的一个锋利的变化。饮食的操作能在如此的变化由断奶应力引起了的 attenuating 起一个重要作用。因此，当当单个精力采购原料在胃肠的 microflora 和 lactobacilli 人口的移动上调查瞬间或 SBP 的效果， inocula， mannan oligosaccharide (瞬间) 或糖甜菜肉(SBP ) 被供应， ileal 和断奶的小猪的结肠的内容被使用。通用 bacteriaand lactobacilli 特定的 PCR/denaturing 坡度胶化电气泳动(DGGE ) ，克隆并且定序技术被使用。通用细菌的 DGGE 侧面证明大变化在发酵以后在主导的乐队的位置，数字和紧张被发现。在回肠和冒号之间的细菌的社区的类似被瞬间或 SBP 处理与 20% 从类似在发酵以后增加到 85-97% 以前发酵。瞬间处理显著地在 ileal 和结肠的发酵增加了细菌的差异和乐队数字(P < 0.05 ) 。SBP 治疗显著地在结肠增加了细菌的差异和乐队数字(P < 0.05 ) 。它暗示某种类被瞬间或 SBP 的增加充实在断奶的小猪增加细菌的社区的类似和差异。五个特定的乐队出现在瞬间或 SBP 处理组在发酵被克隆并且定序以后，与 Prevotella 和 Ruminococcus 有关的种类的变化被观察。与有 98% 类似的未耕作的细菌有关的二个乐队被瞬间或 SBP 处理检测。然而，瞬间或 SBP 处理揭示的类似，差异索引和 lactobacilli 种类上没有效果。这些结果暗示瞬间或 SBP 能在 GIT microflora 由 stablizing microbiota 在断奶的小猪上有有益的效果。

英文摘要：

Microbiota in the gastrointestinal tract （GIT） of piglets during weaning transition can experience a sharp change which could result in growth reduction and diarrhea of weaned piglets. Dietary manipulations can play an important role in attenuating such changes caused by weaning stress. Therefore, ileal and colonic contents of weaned piglets were used as inocula, mannan oligosaccharide （MOS） or sugar beet pulp （SBP） was supplied as single energy sources to investigate effects of MOS or SBP on the shifts of gastro-intestinal microflora and lactobacilli populations. The universal bacteria- and lactobacilli-specific PCR/denaturing gradient gel electrophoresis （DGGE）, cloning and sequencing techniques were used. DGGE profiles of the universal bacteria showed that great changes were found in the position, numbers and intensity of dominant bands after fermentation. The similarity of bacterial community between ileum and colon was increased to 85-97% by MOS or SBP treatment after fermentation from the similarity with 20% before fermentation. MOS treatment significantly increased the bacterial diversity and band number in both ileal and colonic fermentation （P〈0.05）. SBP treatment significantly increased the bacterial diversity and band number in colon （P〈0.05）. It implies that some species were enriched by the addition of MOS or SBP to increase the similarity and diversity of bacterial community in weaned piglets. Five specific bands appearing in MOS or SBP treatment group after fermentation were cloned and sequenced, the changes of species related to Prevotella and Ruminococcus were observed. Two bands related to uncultured bacterium with 98% similarity were detected by MOS or SBP treatment. However, there were no effects on the similarity, diversity index and lactobacilli species revealed by MOS or SBP treatment. These results imply that MOS or SBP could have beneficial effects on the weaning piglets by stablizing microbiota in the GIT microflora.