山羊绒毛的产量和绒毛纤维的质量与角蛋白有关.本研究旨在探讨内蒙古白绒山羊(Capra cashmere)毛囊差异表达蛋白质的变化规律,采用双向凝胶电泳(two dimensional gel electrophoresis,2-DE)技术分离一年中12个月份毛囊总蛋白质,建立了毛囊发育周期蛋白质图谱.使用质谱鉴定差异表达蛋白点,并分析部分蛋白点的表达趋势.结果表明,12个月样本的2-DE图谱显示,平均每块胶上可检测到255个蛋白点,分布在pI 4~7,分子量30~85 kD;软件分析得到20个差异表达蛋白点,质谱成功鉴定出12种蛋白质;这些蛋白质主要为角蛋白(keratin,K),参与细胞生物过程并发挥生物学功能.将12个月做以下划分:11、12、1和2月4个月归为毛囊退行期,3和4月归为毛囊休止期,此时蛋白质表达量明显降低;5、6、7、8、9和10月归为毛囊兴盛期,蛋白质表达量为一年中最高.初步得出K1、K5、K71和K25在毛囊发育的兴盛期表达量高,可能促进毛囊的生长发育.K83和K10在毛囊休止期表达量高,可能与毛囊的休止有关.研究建立了良好的皮肤毛囊蛋白质表达谱,分析并鉴定了差异蛋白质及其表达趋势,对于从蛋白质组学研究内蒙古白绒山羊毛囊生长和发育,寻找标记蛋白应用于绒山羊育种提供了新的研究线索.
Keratin relates to the output of cashmere wool and the quality of fluff fiber. The purpose of this study is to research the change rule of differentially expressed protein of hair follicle in Inner Mongoliacashmere goat(Capra cashmere). Two- dimensional gel electrophoresis(2-DE) was used to separate total protein of hair follicle in 12 months and protein map for hair follicle cycle was established. Differentiallyexpressed protein spots were identified and the expression trends of partial protein spots were analyzed by mass spectrometry. The results indicated that 255 protein spots were distributed at pI 4-7 and their molecularweight were 30-85 kD in each 2-DE maps of 12 months. 20 differentially expressed protein spots were found with Image Master 2-D platinum software. Finally, 12 protein spots were successfully identified by massspectrometry, and they were mainly keratins (K) which participated in cell biological process and playedimportant biological function. The 2-DE maps of 12 months were divided as follows: Four months of November, December, January and February were classified as hair follicle catagen; March and April wereclassified as hair follicle telogen, when proteins expression significantlly reduced; six months of May, June, July, August, September and October were classified as flourishing period with the highest expression.Preliminary analysis results showed that the expression level of K1, K5, K71 and K25 were the highest in flourishing period, which might promote the growth of hair follicle. At the same time, the expression level ofK83 and K10 were the highest in the period of telogen, which would have the relationship with hair follicle telogen. In conclusion, excellent protein expression profiles for hair follicle were established and differentiallyexpressed protein spots were successfully analyzed in this study, which will provide basic data for research on hair follicle cycle based on proteomics for Inner Mongolia Cashmere goat and searching marker protein.